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Fig. 6 | Parasites & Vectors

Fig. 6

From: Cryptosporidium parvum regulates HCT-8 cell autophagy to facilitate survival via inhibiting miR-26a and promoting miR-30a expression

Fig. 6

MiR-26a, miR-30a, ERK signaling and P38 signaling regulated C. parvum proliferation in HCT-8 cells. HCT-8 cells were transfected with miR-30a-mimic, miR-30a-inhibitor, miR-26a-mimic or miR-26a-inhibitor and were infected with C. parvum sporozoites for 24 h. Parasite numbers (A, C) and cell numbers (B, D) were quantified by using qPCR assays. HCT-8 cells were incubated with ERK inhibitor LY3214996 (1 μM) or P38 inhibitor SB203580 (5 μM) for 1 h before being challenged with C. parvum sporozoites for 24 h. Parasite numbers (E) and cell numbers (F) were quantified by using qPCR assays. A one-way ANOVA assay with the Tukey-Kramer post hoc test was used for analyzing data. Data are expressed as the mean ± SD from three independent experiments (*P < 0.05, **P < 0.01, ***P < 0.001)

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