Fig. 2

TgCtwh6 infection leads to the apoptosis of hippocampus cells in mouse. The mouse brain tissues were cut into sections for staining. The hippocampus tissue section was first stained with hematoxylin and eosin, and then the neurons were observed under a light microscope at a magnification of 20 × 100 and 40 × 100 (scale bars, 50 μm and 20 μm, respectively) (A). Besides, the mouse hippocampus tissue section was stained with Nissl dye, and then the neurons were observed under a light microscope at a magnification of 20 × 100 and 40 × 100 (scale bars, 50 μm and 20 μm, respectively). The number of positive cells in each field of view were evaluated with semiquantitative analysis (B, C). The mouse right brain hippocampus tissues were lysed and proteins were extracted; then, the apoptosis-related proteins were assessed by western blotting and the protein expression was determined semiquantitatively (D–G). Synaptotagmin 1 protein from hippocampus tissue was detected by western blotting and analyzed using semiquantitative method (H, I). Data are represented as mean ± SEM and were analyzed by two-tailed Student’s t-test for comparing the difference between the two groups (n = 3 each group). *P < 0. 05, **P < 0.01, ***P < 0.001