Fig. 5

TgCtwh6 tachizoites polarize BV2 to M1 probably via Notch signaling pathway. After BV2 cells were infected by TgCtwh6 tachyzoites for 24 h, BV2 cells were collected and assessed quantitatively for polarization status using FCM (A–C). Additionally, the infected BV2 cells were lysed and proteins extracted. The expression of Notch and Hes1 proteins was detected by western blotting and then analyzed semiquantitatively (D–F). Data were represented as mean ± SEM and analyzed by two-tailed Student’s t-test for comparing the difference between the two groups (n = 3 each group). **P < 0.01, ***P < 0.001