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Fig. 9 | Parasites & Vectors

Fig. 9

From: Studies on the mechanism of Toxoplasma gondii Chinese 1 genotype Wh6 strain causing mice abnormal cognitive behavior

Fig. 9

TgCtwh6 tachyzoites induce Aβ production by activating NF-κB signaling pathway in HT22. HT22 cells pre-treated with or without PDTC for 12 h were challenged with or without TgCtwh6 tachyzoites for 24 h. Then, after the HT22 cells had been collected and lysed, the proteins in HT22 were extracted. The expressions of NF-κBp65, p-NF-κBp65, APP and BACE1 were detected by western blotting and then semiauantitative analyzed (A–E). Additionally, after the HT22 cells had been fixed and the cytomembranes penetrated by Triton, the NF-κBp65, p-NF-κBp65, BACE1 and Aβ proteins were visualized in situ by immunofluorescence staining and were observed under a fluorescence microscope at a magnification of 40 × 100. The percentage of positively stained cells was calculated using morphometric software (F–M). Data were represented as mean ± SEM and were analyzed by one-way ANOVA followed by Bonferroni’s post hoc test for multiple comparisons (n = 3 each group). *P < 0. 05, **P < 0.01, ***P < 0.001

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