Fig. 1
From: High-throughput analysis of the transcriptional patterns of sexual genes in malaria
The robot-automated method yields high purity Pf RNA. a, b Spectrophotometric quantification of the RNA yield extracted from Pf-iRBC ring or trophozoite stage cultures using (i) robot-automated magnetic binding (rMB), (ii) solid phase extraction (SPE) or (iii) liquid–liquid extraction (LLE) methods. c, d Mean A260/A280 purity ratio determined spectrophotometrically for samples extracted with the same methods. e, f Mean RNA integrity number (RIN) calculated by capillary electrophoresis (TapeStation® analysis). Panels a–d present the mean of three biological replicates (n = 3) with three technical replicates each. Panels e, f present the mean of three biological replicates (n = 3) with one technical replicate each. Error bars represent SD. A one-way ANOVA with Tukey’s multiple comparisons test was performed for panels a–d, and a single-value t-test of the mean RIN against the RIN value of 8.5 (considered minimum for high-quality RNA [71], represented as a horizontal line in the plot) was performed for panels e, f. ns = p ≥ 0.05 (non-significant), *p < 0.05, **p ≤ 0.01, ***p ≤ 0.001 and ****p ≤ 0.0001