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Fig. 6 | Parasites & Vectors

Fig. 6

From: High-throughput analysis of the transcriptional patterns of sexual genes in malaria

Fig. 6

Schematic illustration of the robot-automated RT-qPCR platform for monitoring Pf gene expression profiles. The goal of the automated RT-qPCR platform is to simultaneously evaluate multiple conditions that may affect gene expression regulation. First, Pf-iRBCs are cultured under different treatments induced by robot-automated culturing (work in progress), incubation and Giemsa smear monitoring (Step 1). RNA is then extracted from Pf-iRBCs by a completely automated high-throughput method, up to 96 samples simultaneously (Step 2). cDNA is next prepared from up to 96 samples simultaneously by robot-automated RNA dilution and reverse-transcription master mix aliquoting (Step 3). Finally, 384-well RT-qPCR plates are prepared using the robotic system by aliquoting the cDNA solutions and the RT-qPCR reaction mix (Step 4)

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