Fig. 2

Eggs harvested from in vitro culture of A. pegreffii and incubated in seawater at 17 °C in 5% CO₂ atmosphere for 6 days before hatching of L2. Incubation time up to a 12 h, b 24 h, c 48 h, d 72 h, e 96 h, f 120 h, g and h 144 h, and i 168 h, resulting in the L2 hatching from the egg. Note the wrinkled ensheathment of the L1 cuticle. Nuclei of hatching L2 are stained by DAPI. Scale bars: 10 µm