Fig. 3

Alignment of the domain IIS1–S5 fragment of the voltage-gated sodium channel gene (vgsc) of the head louse Pediculus humanus capitis with accession no. OM891554 against the reference vssc gene of wild-type P. humanus capitis (accession no. AY191157). The mutation sites are indicated in differently colored vertical boxes: red depicts the nonsense mutation at M815, T917 and L920 sites; green depicts silent mutations with transversion (at P790 and A822 sites) and transition (at F791 site). Nucleotides (nt) 1–138 include exon I, nt 139–224 include intron I, nt 225–398 include exon II, nt 399–486 include intron II, nt 487–649 include exon III, nt 650–735 include intron II and nt 736–999 include exon IV. NS and AS represent nucleotide and deduced amino acid sequences, respectively. The locations of reverse primers are presented in blue (initial PCR), violet (conventional PCR) and red (SYBR Green real-time PCR) highlights, and the forward primer is marked with a green arrow. The segments, intracellular intersegment linkers and extracellular intersegment linkers of VSSC domain II are highlighted with blue, yellow, and green, respectively