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Fig. 1 | Parasites & Vectors

Fig. 1

From: Range of metastrongylids (superfamily Metastrongyloidea) of public health and veterinary concern present in livers of the endemic lizard Gallotia galloti of Tenerife, Canary Islands, Spain

Fig. 1

Representative result of the multiplex-nested PCR of DNA-spiked samples showing the identifying/differentiation power of the assay in a 2% agarose gel with 1-kb Plus DNA ladder molecular weight marker (New England Biolabs, Ipswich, MA, USA). In terms of size of the respective products, the 642-bp band represents Angiostrongylus cantonensis, the 537-bp band represents Aelurostronylus abstrusus, the 392-bp band represents Angiostrongylus vasorum and the 377-bp band represents Crenosoma striatum; in the case of unidentified metastrongylid, an additional band around 300 bp appeared (lanes 4, 6). Lanes: 1 A. cantonensis + Ae. abstrusus + C. striatum; 2 A. cantonensis + Ae. abstrusus + A. vasorum + C. striatum; 3 Ae. abstrusus + A. vasorum + C. striatum, 4, 6 A. cantonensis + unidentified metastrongylid; 5, 7 A. cantonensis + A. vasorum + C. striatum; 8, 9 C. striatum only

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Parasites & Vectors

ISSN: 1756-3305

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