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Table 1 Primers designed for the multiplex-nested PCR analysis and the molecular weights of the resulting products

From: Range of metastrongylids (superfamily Metastrongyloidea) of public health and veterinary concern present in livers of the endemic lizard Gallotia galloti of Tenerife, Canary Islands, Spain

PCR Round

Primer

Sequence (5′–3′)

Product size (bp)a

Speciesa

1

ITS1_F1674

GTCGTAACAAGGTATCTGTAGGTG

  

ITS1_58SR4

TAGCTGCGTTTTTCATCGATA

2

ITS1_Canto_F3

AACAACTAGCATCATCTACGTC

642

Angiostrongylus cantonensis

ITS1_Canto_R1

CATCCTGTGTATCTCGTTCC

ITS1_Aeluro_F1

GCTTTGATCAACGAGAAACC

537

Aelurostronylus abstrusus

ITS1_Aeluro_R2

CATACGTGCACAGTATAATCTC

ITS1_Vasor_F1

CTCATCGTCATCATCGTTATAG

492

Angiostrongylus vasorum

ITS1_Vasor_R1

ACCATATTCAGTAGTCATTGTC

ITS1_Creno_s_R2

GTACCACGTAACACACGA

377 

Crenosoma striatum

ITS1_Creno_F2

TCTGGAATTTTTGTGGATTGG

ITS1_Creno_v_R1

GCTACTTATCAAGTAAGCTAGC

299

Crenosoma vulpis

  1. ITS1 Internal transcribed spacer 1
  2. aDetection method for C. striatum and C. vulpis share the forward primer