Table 1 Dogs included in the study divided according to their positivity/negativity to Dirofilaria immitis and Leishmania infantum, microfilariae (mfs) load, IFAT titer and positivity for Wolbachia and L. infantum by qPCR
From: Interaction between Wolbachia pipientis and Leishmania infantum in heartworm infected dogs
Groups | Dogs | Knott test mfs load | Snap test | qPCR Wolbachia | IFAT L. infantum n dogs; titer | qPCR L. infantum | |||
|---|---|---|---|---|---|---|---|---|---|
n dogs Pos (%; 95% CI) | Ct | n dogs Pos (%; 95% CI) | Ct | ||||||
min–mean–max | min–mean–max | ||||||||
G1 | Dim + /Leis + | 18 | 5 < low ≤ 200 | Na | 11 (61.1; 0.357–0.827) | 29.2–33.1–37.1 | n = 7; 1:80 | 1 (5.5; 0.001–0.273) | 37.8 |
n = 2; 1:160 | |||||||||
n = 4; 1:320 | |||||||||
n = 3; 1:640 | |||||||||
n = 2; 1:2560 | |||||||||
10 | 200 < medium ≤ 300 | 7 (70; 0.348–0.933) | 26.6–31.7–35.3 | n = 7; 1:80 | 1 (10; 0.003–0.445) | 33.9 | |||
n = 2; 1:320 | |||||||||
n = 1; 1:640 | |||||||||
19 | High ≥ 600 | 19 (100; 0.824–1.000) | 22.1–29.5–35.4 | n = 11; 1:80 | 4 (21; 0.061–0.456) | 24.7–30.7–36.5 | |||
n = 4; 1:160 | |||||||||
n = 2; 1:320 | |||||||||
n = 2; 1:1280 | |||||||||
Total | 47 | 37 (78.7; 0.643–0.893) | 6 (12.8; 0.048–0.257) | – | |||||
G2 | Dim + /Leis− | 20 | 1 < low ≤ 200 | Na | 16 (75; 0.563–0.943) | 28.8–32.9–37.4 | Neg | Neg | Na |
11 | 200 < medium ≤ 300 | 11 (100; 0.715–1.000) | 28.3–30.6–32.8 | ||||||
15 | high ≥ 400 | 15 (100; 0.782–1.000) | 28.4–31.1–35.6 | ||||||
Total | 46 | – | 42 (91.3; 0.792–0.976) | – | – | – | |||
G3 | Dim −/Leis + | Neg | Neg | Na | Na | n = 15; 1:80 | 2 (4.2; 0.005–0.145) | 32.59–32.8–33.1 | |
n = 9; 1:160 | |||||||||
n = 6; 1:320 | |||||||||
n = 8; 1:640 | |||||||||
n = 6; 1:1280 | |||||||||
n = 3; 1:2560 | |||||||||
Total | 47 | – | – | – | – | – | 2 (4.2; 0.005–0.145) | – | |
G4 | Dim −/Leis − | 47 | Neg | Neg | Na | Na | Neg | Neg | Na |
Total | 187 | – | 79 (85; 0.760–0.915) | 8 (4.3; 0.019–0.083) | |||||