Table 3 Prevalence of Wolbachia in dogs positive for Dirofilaria immitis divided according to the microfilaria load and the range of Ct value of qPCR
From: Interaction between Wolbachia pipientis and Leishmania infantum in heartworm infected dogs
Groups | Knott test | n dogs | qPCR Wolbachia | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
mfs load | Ct = 0 | Prevalence (%); 95% CI | 25 ≤ Ct ≤ 30 | Prevalence (%); 95% CI | 30 < Ct ≤ 34 | Prevalence (%); 95% CI | Ct > 35 | Prevalence (%); 95% CI | |||
G1 | Dim + /Leis + | 5 ≤ low ≤ 200 | 18 | 7 | 38.9; 0.173–0.643 | 2d | 11.1; 0.014–0.347 | 4 | 22.2; 0.064–0.476 | 5 | 27; 0.097–0.535 |
200 < medium ≤ 300 | 10 | 3 | 30; 0.067–0.652 | 3 | 30; 0.067–0.652 | 1 | 10; 0.003–0.445 | 3 | 30; 0.067–0.652 | ||
High ≥ 600 | 19 | 0 | – | 12c,d | 63.2; 0.384–0.837 | 5c | 26.3; 0.091–0.51 | 2c | 30; 0.067–0.652 | ||
Tot | 47 | 10 | 21.3; 0.107–0.357 | 17 | 36.2; 0.227–0.515 | 10 | 21.3; 0.107–0.357 | 10 | 21.3; 0.107–0.357 | ||
G2 | Dim + /Leis− | 1 ≤ low ≤ 200 | 20 | 5 | 25%; 0.087–0.491 | 5 | 25; 0.087–0.491 | 6 | 30; 0.119–0.543 | 4 | 20; 0.057–0.437 |
200 < medium ≤ 300 | 11 | 0 | – | 5 | 54.5; 0.234–0.833 | 6 | 54.5; 0.234–0.833 | 0 | 0 | ||
High ≥ 400 | 15 | 0 | – | 8e | 53.3; 0.266–0.787 | 6e | 40; 0.163–0.677 | 1e | 6.7; 0.002–0.319 | ||
Tot | 46 | 5 | 10.9; 0.036–0.236 | 18 | 39.1; 0.251–0.546 | 18 | 39.1; 0.251–0.546 | 5 | 10.9; 0.036–0.236 | ||
Total | 93 | 15 | 16.1; 0.093–0.252 | 35a | 37.6; 0.278–0.483 | 28b | 30.1; 0.210–0.405 | 15a,b | 16.1; 0.093–0.252 | ||