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Fig. 1 | Parasites & Vectors

Fig. 1

From: A proteasomal β5 subunit of Haemonchus contortus with a role in the growth, development and life span

Fig. 1

Hc-PBS-5 displays conserved features with PBS-5 homologs from selected species. a The rooted neighbor-joining tree showing the phylogenetic relationships of Haemonchus contortus proteasomal β5 subunit (Hc-PBS-5) with other proteasomal β5 subunit homologs, whose sequences are listed in Additional file 1: Table S1. The Saccharomyces cerevisiae proteasome core particle subunit beta 1 was set as an outgroup. Their corresponding accession numbers are given to the right of each species name. The tree was built employing the Jones-Taylor-Thornton (JTT) model, and confidence limits were assessed using a bootstrap procedure employing 1000 pseudo-replicates. Nodal support values for each clade are color coded. b Amino acid sequence alignment of Hc-PBS-5 and other selected species, whose sequences are listed in Additional file 1: Table S1. Secondary structure elements were identified with reference to Saccharomyces cerevisiae PBS-5 and labeled above the sequence with α-helices represented with orange cylinders and β-strands represented with orange arrows. Conserved sequences were indicated in red squares; protease active core and bortezomib (BTZ) binding sites in purple and BTZ binding site in cyan and protease active cores in green. (c) Putative three-dimensional structure of Hc-PBS-5 was generated using the SWISS-MODEL server. In the putative three-dimensional structure, α helixes are marked in orange, β strands are in yellow, and the S1 (‘specificity’) pocket is marked with a white box. Important amino acids are also color coded: protease active core and bortezomib (BTZ) binding site Thr64 is in red, Met115 located in the bottle of the specific S1 pocket is in pink, BTZ binding sites are in purple, and protease active cores are in blue

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