Nanopore adaptive sampling for targeted mitochondrial genome sequencing and bloodmeal identification in hematophagous insects

Table 1 Individual nanopore sequencing experiments conducted to sequence genomic DNA from five blood-fed insects

Experiment ID	NAS technique	Species	Run time	Total bases	Number of reads (in millions)	Average read length (N50) per experiment (in bp)^a
A^b	Enriched using user-generated reference file	Chrysops niger	22 h 42 m	600 Mb	1.5	401
B^b	Enriched using user-generated reference file	Culex restuans	45 h 47 m	1 Gb	2.5	462
B^b	Enriched using user-generated reference file	Culex territans	45 h 47 m	2 Gb	4.5	462
C^b	Enriched using mitogenome database as reference	Aedes vexans	21 h 7 m	4 Gb	10.8	536
D	Control run: no NAS	Culex restuans	44 h 58 m	5 Gb	6.2	1960
E	Enriched using user-generated reference file	Culex restuans	27 h 15 m	350 Mb	1.0	347
E^b	Enriched using user-generated reference file	Aedes trivittatus	27 h 15 m	200 Mb	0.6	347

NAS Nanopore adaptive sampling
NCBI accession numbers for NAS enrichment files are provided in Additional file 2: Table S2
^aN50 metric: sequence length of the shortest contig at 50% of the total assembly length
^bData from these experiments were used for downstream insect mitogenome assemblies

ISSN: 1756-3305