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Table 1 Primer pairs and PCR conditions used for PCR amplification

From: Apicomplexan haemoparasites in domestic cats in Romania

Target and genetic marker

Primer name and sequence (5′–3′)

Annealing temperature/amplicon length

Reference

Piroplasmida 18S rDNA

BTH_F: CCTGMGARACGGCTACCACATCT

60 °C/686–747 bp

[91]

BTH_R: TTGCGACCATACTCCCCCCA

GF2: GTCTTGTAATTGGAATGATGG

50 °C/561–613 bp

[92, 93]

GR2: CCAAAGACTTTGATTTCTCTC

Babesia spp. 18S rDNA

Bc_F1: CGTAGTTGTATTTTTGCGT

50 °C/≈ 430 bp

[94]

GR2: CCAAAGACTTTGATTTCTCTC

Bc_F2: CATTTGGTTGGTTATTTCGTTTT

53 °C/376 bp

Bc_R1: GTTCCTGAAGGGGTCAAAAA

Babesia spp. Cytb

Bc_cytb_F1: TGGTCWTGGTATTCWGGAATG

50 °C/≈ 700 bp

[95]

Bc_cytb_R1: AAGMYARTCTYCCTAAACATCC

Bc_cytb_F2: RATKAGYTAYTGGGGAGC

48 °C/≈ 580 bp

Bc_cytb_R2: GCTGGWATCATWGGTATAC

Babesia spp. COI

Bab_For1: ATWGGATTYTATATGAGTAT

45 °C/1250 bp

[95]

Bab_Rev1: ATAATCWGGWATYCTCCTTGG

Bab_For2: TCTCTWCATGGWTTAATTATGATAT

49 °C/980 bp

Bab_Rev2: TAGCTCCAATTGAHARWACAAAGTG

Cytauxzoon spp. 18S rDNA

7549F: GTCAGGATCCTGGGTTGATCCTGCCAG

60 °C/1726 bp

[64]

7548R: GACTGAATTCGACTTCTCCTTCCTTTAAG

Cyt-SSU-F2: CATGGATAACCGTGCTAATTG

53 °C/1335 bp

Cyt-SSU-R4: AGGATGAACTCGATGAATGCA

Hepatozoon spp. 18S rDNA

HAM1F: GCCAGTAGTCATATGCTTGTC

52 °C/≈ 1700 bp

[95]

HPF2R: GACTTCTCCTTCGTCTAAG

EF-M: AAAACTGCAAATGG CTCATT

55 °C/≈ 1600 bp

Hep1615R: AAAGGGCAGGGACGTAATC