From: Apicomplexan haemoparasites in domestic cats in Romania
Target and genetic marker | Primer name and sequence (5′–3′) | Annealing temperature/amplicon length | Reference |
---|---|---|---|
Piroplasmida 18S rDNA | BTH_F: CCTGMGARACGGCTACCACATCT | 60 °C/686–747 bp | [91] |
BTH_R: TTGCGACCATACTCCCCCCA | |||
GF2: GTCTTGTAATTGGAATGATGG | 50 °C/561–613 bp | ||
GR2: CCAAAGACTTTGATTTCTCTC | |||
Babesia spp. 18S rDNA | Bc_F1: CGTAGTTGTATTTTTGCGT | 50 °C/≈ 430 bp | [94] |
GR2: CCAAAGACTTTGATTTCTCTC | |||
Bc_F2: CATTTGGTTGGTTATTTCGTTTT | 53 °C/376 bp | ||
Bc_R1: GTTCCTGAAGGGGTCAAAAA | |||
Babesia spp. Cytb | Bc_cytb_F1: TGGTCWTGGTATTCWGGAATG | 50 °C/≈ 700 bp | [95] |
Bc_cytb_R1: AAGMYARTCTYCCTAAACATCC | |||
Bc_cytb_F2: RATKAGYTAYTGGGGAGC | 48 °C/≈ 580 bp | ||
Bc_cytb_R2: GCTGGWATCATWGGTATAC | |||
Babesia spp. COI | Bab_For1: ATWGGATTYTATATGAGTAT | 45 °C/1250 bp | [95] |
Bab_Rev1: ATAATCWGGWATYCTCCTTGG | |||
Bab_For2: TCTCTWCATGGWTTAATTATGATAT | 49 °C/980 bp | ||
Bab_Rev2: TAGCTCCAATTGAHARWACAAAGTG | |||
Cytauxzoon spp. 18S rDNA | 7549F: GTCAGGATCCTGGGTTGATCCTGCCAG | 60 °C/1726 bp | [64] |
7548R: GACTGAATTCGACTTCTCCTTCCTTTAAG | |||
Cyt-SSU-F2: CATGGATAACCGTGCTAATTG | 53 °C/1335 bp | ||
Cyt-SSU-R4: AGGATGAACTCGATGAATGCA | |||
Hepatozoon spp. 18S rDNA | HAM1F: GCCAGTAGTCATATGCTTGTC | 52 °C/≈ 1700 bp | [95] |
HPF2R: GACTTCTCCTTCGTCTAAG | |||
EF-M: AAAACTGCAAATGG CTCATT | 55 °C/≈ 1600 bp | ||
Hep1615R: AAAGGGCAGGGACGTAATC |