Fig. 2

β-Glucan administration ameliorated neurite degeneration and the decrease in dendritic spine density in the prefrontal cortex (PFC) of chronic T. gondii Wh6-infected mice. Variations in neuronal morphology were analyzed by Golgi-Cox staining. a Representative images of pyramidal neurons in the PFC of mice. Scale bars: 100 μm. b Total neurite length per cell. c Number of neurite branches. d Average neurite length per branch. e The sum intersection of neuron. f The max intersection distance from soma (n = 10/group). g Sholl analysis of dendritic branching complexity of pyramidal neurons in the PFC of mice. h Representative images of dendritic spines of neurons in the PFC. Scale bars: 10 μm. The sum intersection of neurons. i Spine density. Sholl analysis of dendritic branching complexity of pyramidal neurons in the PFC of mice. Values are presented as the mean ± SEM. Asterisks indicate a statistically significant difference at *P < 0.05, ** P < 0.01, *** P < 0.001, according to one-way ANOVA followed by Tukey’s test for multiple comparisons