Fig. 6
From: β-Glucan alleviates goal-directed behavioral deficits in mice infected with Toxoplasma gondii
β-Glucan administration suppressed the expression of pro-inflammatory cytokines in the PFC of chronic T. gondii Wh6-infected mice. Double immunofluorescence staining was performed to detect the IL-6 expression in the microglia and astrocyte of chronic T. gondii Wh6-infected mice. a Representative double immunofluorescence staining of Iba1 (red) and IL-6 (green) in the PFC of mice. The white arrowheads point to Iba1+IL-6-positive (IL-6+) cells. Scale bar: 50 μm or 20 μm. b Percentage of Iba1+IL-6+ cells in Iba1+ cells in the PFC. c Quantification of the mean fluorescence intensity of IL-6+ cells in the PFC. d Percentage of GFAP+IL-6+ cells in GFAP+ cells in the PFC. e Quantification of the mean fluorescence intensity of GFAP+ cells. f Representative double immunofluorescence staining of GFAP (red) and IL-6 (green) in the PFC of mice. Scale bar: 50 μm or 20 μm. g Real-time reverse transcription PCR (RT-PCR) analysis of IL-6 mRNA expression in the PFC. h RT-PCR analysis of TNF-α mRNA expression in the PFC. i RT-PCR analysis of IL-1β mRNA expression of in the PFC. j Cyst numbers in the brain of chronic T. gondii Wh6-infected mice. Values are presented as the mean ± SEM. Asterisks indicate a statistically significant difference at *P < 0.05, ** P < 0.01, *** P < 0.001, according to one-way ANOVA followed by Tukey’s test for comparisons among four groups and the unpaired t-test for comparisons between two groups. IL, Interleukin; mRNA, messenger RNA; TNF-α, tumor necrosis factor alpha