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Fig. 1 | Parasites & Vectors

Fig. 1

From: Clonorchis sinensis legumain promotes migration and invasion of cholangiocarcinoma cells via regulating tumor-related molecules

Fig. 1

The basic information of Cslegumain. a Predicted tertiary structure of Cslegumain. Cslegumain showed a very similar tertiary structure as Hlegumain, with a catalytic AEP domain (in the green frame) and a C-terminal prodomain composed of AP domain and LSAM domain. b The tertiary structure of Hlegumain (Elfriede et al., 2013). c Purified recombinant Cslegumain from E. coli. The arrow shows the purified protein band. M, marker; lane 1, uninduced; lane 2, induced total; lane 3, supernatant; lane 4, pellet; lane 5, pass through; lane 6, washing; lanes 7–12, eluted and purified Cslegumain. (d) Detection of recombinant and native Cslegumain. Lanes 1–4, immunized mouse sera recognized recombinant Cslegumain; lane 5, normal mouse sera did not react with the recombinant protein. Lanes 6–9, immunized mouse sera recognized native Cslegumain; lane 10, normal mouse sera did not react with native Cslegumain

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Parasites & Vectors

ISSN: 1756-3305

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