Fig. 6

Effects of Cslegumain on expression levels of tumor-related molecules. a Relative transcriptional levels of the tumor-related molecules analyzed by qRT-PCR. Values were compared using one-way ANOVA. Compared with pcDNA3.1(+)-transfected and control RBE cells, Cslegumain-expressing cells displayed decreased transcriptional levels of E-cadherin and N-cadherin as well as increased α-actinin4, β-catenin and iNOS 48 h after culture. However, the levels of PI3K and AKT showed no significance among the three groups (*P < 0.05; **P < 0.01; ***P < 0.001). b Effects of Cslegumain on protein levels of E-cadherin, N-cadherin, actinin4 and β-catenin. The results indicated that Cslegumain downregulated the protein levels of E-cadherin and N-cadherin and upregulated the level of actinin 4 and β-catenin