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Fig. 1 | Parasites & Vectors

Fig. 1

From: Development of environmental loop-mediated isothermal amplification (eLAMP) diagnostic tool for Bulinus truncatus field detection

Fig. 1

Specificity and LOD (limit of detection) of the Bulinus truncatus eLAMP (Bt-eLAMP) assay. Results obtained with agarose gel electrophoresis after 45 min of reaction. a Specificity; lanes 1–5, Bulinus truncatus DNA templates from five different localities (Diama, Lampsar, Mbane, Corsica and Saneinte); lane 6, Bulinus globosus; lane 7, Bulinus senegalensis; lane 8, Bulinus forskalli; lane 9, Bulinus umbilicatus; lane 10, Biomphalaria glabrata; lane 11, Bithynia tentaculata; lane 12, Ancylus fluviatilis; lane 13, Gyraulus laevis; lane 14, Physa acuta; lane 15, Potamopyrgus antipodarum; lane 16, Pisidium casertanum; lane C + , positive control (Bulinus truncatus DNA; 1 ng); lane C-, negative control (water as template). b LOD; lane 1–11, Bulinus truncatus DNA tenfold serial dilutions (10 ng/μL to 1 ag/μl); lane C-, negative control (water as template)

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Parasites & Vectors

ISSN: 1756-3305

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