Fig. 2

Comparison of Bulinus truncatus environmental DNA detection by loop-mediated isothermal amplification (LAMP), quantitative polymerase chain reaction (qPCR) and droplet digital polymerase chain reaction (ddPCR) amplification methods. Each cell represents DNA amplification results from eDNA collected in two Corsican rivers (Cavu and Solenzara) and Perpignan Channel; Bulinus truncatus snails are present in all sites except the water intake site in the Cavu River and university site in the Perpignan Channel. Three volumes of water (1, 3 and 5 l) from both the shore and the streambeds of the rivers were collected in all Corsican sites. Each line represents the amplification result from each site using LAMP, qPCR or ddPCR amplification method. qPCR and ddPCR results come from Mulero et al. [21]. A green membrane means that at least one quarter of the filtration membrane is positive for Bulinus truncatus presence and red membrane means that no quarter of the filtration membrane is positive. Empty cells mean that no filtration was done under these conditions