Table 2 Primer sequences designed for Bulinus truncatus eLAMP assays
Target | Primers | Sequences (5’-3’) | Sequence length (bp) | Melting temperature (°C) |
|---|---|---|---|---|
Internal transcribed spacer 2 (ITS2) | F3 | GTCCTTCCTGTCCCCCTT | 18 | 59.79 |
B3 | CGTCACATGTCCACCTCACA | 20 | 61.59 | |
F2 | ACGCATGTCGCCGTTAC | 17 | 59.33 | |
F1c | AAGAGCACGAGCCGGGTCC | 19 | 66.28 | |
B2 | CGCGTTATAGCCCATCTCAA | 20 | 59.37 | |
B1c | GCGAGTCATACGGACCTCGTG | 21 | 64.21 | |
LB | CACAGTCTCTCCCCCACACC | 20 | 63.67 | |
LF | CCTGGCGACGAGAGATCACC | 20 | 63.89 |