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Table 1 Detection of Mayaro virus using RT-qPCR in pools of female Mansonia humeralis

From: Report of natural Mayaro virus infection in Mansonia humeralis (Dyar & Knab, Diptera: Culicidae)

RT-qPCR MAYV

RT-qPCR MAYV

Pool number

Cq

Number of copies/µl

Pool number

Cq

Number of copies/µl

2

34.47

10

25

33.82

16

3

33.45

19

26

33.61

18

4

31.59

61

28

27.39

821

5

33.05

25

30

30.74

104

6

32.90

27

31b

28.69

368

8

29.30

253

32

33.43

20

10

32.69

31

34

33.40

20

12

31.58

62

41

31.19

79

14

30.45

124

42

31.24

76

15

33.92

15

43c

29.14

279

18

34.71

9

45

34.74

9

19

34.53

10

46d

28.88

327

20

30.90

94

48

25.73

2287

21

32.12

44

49

34.41

11

22a

26.27

1639

50

30.71

106

23

30.26

140

57

34.58

10

24

33.02

25

184

26.82

1167

  1. a–dThese pools were used for the in vitro infection experiment