Fig. 5
From: Identification and characterisation of the haemozoin of Haemonchus contortus
Haemozoin-like pigments form in L4s of in vitro culture. Based on previous culture conditions of L4s in vitro, additional 12.5% deflbrinated blood (DFB) from goat was added. A, B At 48 h of cocultivation, haemozoin-like pigments were microscopically observed in L4s cultured with/without DFB. Scale bar = 40 μm. C Wavelengths of haemozoin were determined (300–700 nm) in L4s cultured in normal medium and with/without DFB. D After 24 h, 48 h and 72 h of cocultivation, the wavelength of haemozoin was spectrophotometrically determined at 400 nm. E Based on normal culture conditions of L4s in vitro, an additional 107, 108 and 109 red blood cells (RBC) were supplemented. The wavelength of haemozoin was determined at 72 h of cocultivation. Data were from three independent replicates. Statistical significance was determined by one-way ANOVA and is indicated by asterisks, *p < 0.033 and **p < 0.002