Fig. 5

Multiple-loci sequence alignment analysis (MLSA) and phylogenetic inference of Wolbachia haplotypes resulting from combining three genes (wsp, FtsZ and 16 s rRNA) detected from mosquitoes in Hainan Province. Phylogenetic inference was performed using the UPGMA method. The percentage of replicate trees (> 50) in which the associated haplotypes clustered together in the bootstrap test (1000 replicates) is shown next to each branch. The evolutionary distances were computed using the Kimura two-parameter method; units are the number of base substitutions per site. Pink dots indicate individuals infected with wAlbA strain, while blue dots indicated individuals infected wAlbB strain, determined by both wsp gene alone and combining with FtsZ and 16 s rRNA sequencing in Aedes albopictus