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Fig. 1 | Parasites & Vectors

Fig. 1

From: Histological confinement of transglutaminase-mediated nit sheath crosslinking is essential for proper oviposition and egg coating in the human head louse, Pediculus humanus capitis

Fig. 1

Histological sites of LNSP1, LNSP2 or TG transcription in the accessory glands and uterus. Representative microscopic images of the accessory glands and uterus of head louse females following in situ hybridization with LNSP1 (a), LNSP2 (b) or TG (c) probe. a, b FITC-labeled probes were used for LNSP1 and LNSP2. Both LNSP1 and LNSP2 were mainly detected in the entire areas of accessory gland with reduced expression being detected in the uterus (green signal). Images of nuclear staining (blue signal) and LNSP1 or LNSP2 were merged. c For TG, DIG-labeled probes were used for signal amplification. TG was exclusively expressed at the focal area around the opening of posterior oviduct (dark purple). A dotted circle indicates the opening of posterior oviduct where an egg passes through for oviposition. ag accessory gland, vu ventral end of uterus, du dorsal end of uterus, po posterior oviduct

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