Fig. 2

Encapsulating eggs for storage at 18 °C does not improve egg fitness compared to 22 °C. Eggs were packaged into water-soluble capsules with larval food or left on paper substrate as a control and stored at 18 °C or 22 °C (control) for 0, 2, 3, 4, 5, 6, 7 or 8 weeks. a Hatch rate b emergence rate and c Wolbachia densities were measured. Each data point represents one cup of 150 mosquitoes (hatch and emergence) or one mosquito (Wolbachia density); 24–40 mosquitoes were sampled for each Wolbachia density group. Hatch rate data were analysed by ANOVA followed by Tukey’s multiple comparison test (not significant [ns], P < 0.01**) and data are shown as the mean and standard error. The secondary significance bars compare hatch rate over time. Emergence rate and Wolbachia density data were analysed by generalised linear model and Kruskal-Wallis H-test (P < 0.05*, P < 0.001***) and data are shown as medians with interquartile ranges. Emergence rate secondary significance bars indicate change over time. Wolbachia density secondary significance bars compare week 8 to the corresponding week 0 control