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Fig. 3 | Parasites & Vectors

Fig. 3

From: Enhancing the scalability of Wolbachia-based vector-borne disease management: time and temperature limits for storage and transport of Wolbachia-infected Aedes aegypti eggs for field releases

Fig. 3

Impact of low egg storage temperatures on egg viability and adult Wolbachia density. WT, wMel and wAlbB-infected eggs on paper substrate were stored at 26 °C, 12 °C and 4 °C for 0, 8 or 48 h. a–c Hatch rate and d-e Wolbachia density were measured. These data are representative of two combined experimental replicates. Each data point represents the average of three cups of 150–300 mosquitoes (hatch rate) or one mosquito (Wolbachia density); 80 mosquitoes were sampled for each Wolbachia density group. Hatch rate data were analysed by ANOVA followed by Tukey’s multiple comparison test (not significant [ns]) to compare changes in hatch rate over time within each group. Data are shown as the mean and standard deviation. Wolbachia density data were analysed by Kruskal-Wallis H-test and Wilcoxon signed rank test (P < 0.05*, P < 0.01**, P < 0.0001****) and data are shown as median with interquartile range

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Parasites & Vectors

ISSN: 1756-3305

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