Fig. 6a, b

Association of ZC3H41/Z41AP with target mRNAs under nutritional stress. a Cells expressing TAP-ZC3H41 were incubated in normal growth medium or in phosphate-buffered saline for 3 h, and association with the indicated transcripts was assessed by RIP followed by quantitative RT-PCR. NT8 transcript was used as a non-bound control. Percentages of immunoprecipitated RNA under starvation stress relative to those measured in normal conditions are expressed as the mean (horizontal lines) ± SEM (shaded areas) of four independent RIPs (dots). The horizontal dashed line indicates 100% binding in starvation relative to normal growth (i.e. no changes in binding). b ZC3H41 and Z41AP levels are unaltered in starved cells. Double-tagged cells expressing both 4xTy-ZC3H41 and TAP-Z41AP were subjected to starvation as described above. Total ZC3H41 or Z41AP levels, as well as those of ZC3H41 associated with Z41AP upon TAP purification, were assessed by immunoblot using antibodies against the tags. α-tubulin served as a loading control for total levels. For other abbreviations, see Figs. 1, 2, 4 and  5