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Table 2 Results of qPCR, IFA-testing, hematological analysis and SAA evaluation as well as treatment in a 6-year-old female spayed European Shorthair cat imported from Spain to Germany infected with Leishmania infantum from day 0 to day 288

From: Feline leishmaniosis with focus on ocular manifestation: a case report

Day

Leishmania qPCRA

(/ml blood)

Leishmania spp. IFATB

≤ 1:32 negative

Serum amyloid AC

< 6.7 µg/l

HematologyD

Treatment

0

768

1:4096

77.5

Mild reticulocytosis; mild leukocytosis with lymphocytosis, monocytosis and eosinopenia; mild thrombocytopenia

Allopurinol 12.5 mg BID p.o.1

Meloxicam 0.05 mg/kg SID p.o.2

48

191

1:512

35.2

Mild non-regenerative anemia; mild leukocytosis with monocytosis

Allopurinol 12.5 mg BID p.o.1

Meloxicam 0.05 mg/kg SID p.o.2

66

1

1:1024

21.4

Mild regenerative anemia

Allopurinol 12.5 mg BID p.o.1

Meloxicam 0.05 mg/kg SID p.o.2

75

Allopurinol 12.5 mg BID p.o.1

Meloxicam 0.05 mg/kg SID p.o.2

Prednisolone acetate3 after healing of the corneal erosion) and Ketorolac-Trometamol4 1 drop BID in each eye

96

238

1:1024

15.2

Mild regenerative anemia; mild leukocytosis with lymphocytosis and monocytosis

Allopurinol 25 mg BID p.o.1

Meloxicam 0.05 mg/kg SID p.o.2

Prednisolone acetate3, Ketorolac-Trometamol4 and Nepafenac5 1 drop BID in each eye

117

94

1:512

13.6

Allopurinol 25 mg BID p.o.1

Meloxicam 0.05 mg/kg SID p.o.2

133

1:256

Mild leukocytosis with monocytosis and eosinopenia

Allopurinol 25 mg BID p.o.1

Meloxicam 0.05 mg/kg SID p.o.2

Ophthalmological treatment protocolE

196

11

1:256

5.9

Mild non-regenerative anemia, mild monocytosis

Allopurinol 25 mg BID p.o.1

Meloxicam 0.05 mg/kg SID p.o.2

Ophthalmological treatment protocolE

288

49

1:256

12.0

Mild leukocytosis with monocytosis and eosinopenia

Allopurinol 25 mg BID p.o.1

Meloxicam 0.05 mg/kg SID p.o.2

Enucleation due to uveitis, glaucoma, and blindness

  1. IFAT immunofluorescence antibody test, qPCR quantitative polymerase chain reaction
  2. Aquantitative TaqMan real-time PCR (target: kinetoplast minicircle DNA)
  3. BMegaFLUO® LEISH, MegaCor Diagnostik GmbH, Hörbranz, Austria; > 1:64 positive
  4. CCobas 8000, Roche
  5. DADVIA 2120i, Siemens Healthineers
  6. ERight eye: chloramphenicol 1 drop TID (Cefenicol® CA 5 mg/ml, CP-Pharma, Burgdorf, Germany), ketorolac trometamol4 1 drop SID, Sodiumhyaluronat and dexpanthenol 1 drop SID (Hyalopanthen® for ocular use, CP-Pharma, Burgdorf, Germany); left eye: nepafenac5, Pred forte AT, sodiumhyaluronat and dexpanthenol 1 drop SID (Hyalopanthen® for ocular use, CP-Pharma, Burgdorf, Germany), tropicamid 1 drop BID (Mydriaticum Stulln® UD for ocular use, Pharma Stulln GmbH, Stulln, Germany)
  7. 1Allopurinol-ratiopharm® 100 mg tablets, ratiopharm, Ulm, Germany
  8. 2Metacam® 0.5 mg/ml oral suspension, Boehringer Ingelheim Vetmedica GmbH, Ingelheim, Germany
  9. 3Inflanefran® forte 10 mg/ml for ocular use, AbbVie Deutschland GmbH & Co. KG, Ludwigshafen am Rhein, Germany
  10. 4Acular® 5 mg/ml for ocular use, AbbVie Deutschland GmbH & Co. KG, Ludwigshafen am Rhein, Germany
  11. 5Nevanac® 1 mg/ml for ocular use, NOVARTIS Pharma GmbH, Nürnberg, Germany