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Fig. 2 | Parasites & Vectors

Fig. 2

From: Examination of gametocyte protein 22 localization and oocyst wall formation in Eimeria necatrix using laser confocal microscopy and scanning electron microscopy

Fig. 2

Laser confocal microscopy (LCM) of WFBs and macrogametocytes. A–D Immunofluorescence co-staining of EnGAM22 and EnGAM59 in WFBs. A Bright-field images of the WFB-rich extract. B Fluorescent micrographs of the WFBs incubated with rabbit anti-rEnGAM22 pAb (visualized with FITC, green). C Fluorescent micrographs of the WFBs incubated with mouse anti-rEnGAM59 pAb (visualized with Cy3, red). D Merged image of (B) and (C). This shows that the enriched fractions include both intact WFB1s and WFB2s. E–H Immunofluorescence co-staining of EnGAM22 and EnGAM59 in macrogametocytes. E Bright-field images of the macrogametocytes. F Fluorescent micrographs of the macrogametocytes incubated with rabbit anti-rEnGAM22 pAb (visualized with FITC, green). G Fluorescent micrographs of the macrogametocytes incubated with mouse anti-rEnGAM59 pAb (visualized with Cy3, red). H Merged image of (F) and (G). I–L Immunofluorescence co-staining of EnGAM22 and WFB proteins in macrogametocytes. I Bright-field images of the macrogametocytes. J Fluorescent micrographs of the macrogametocytes incubated with rabbit anti-rEnGAM22 pAb (visualized with FITC, green). K Fluorescent micrographs of the macrogametocytes incubated with mouse anti-WFB pAb (visualized with Cy3, red). L Merged image of (J) and (K). M–P Immunofluorescence co-staining of EnGAM59 and WFB proteins in macrogametocytes. M Bright-field images of the macrogametocytes. N Fluorescent micrographs of the macrogametocytes incubated with rabbit anti-rEnGAM59 pAb (visualized with FITC, green). (O) Fluorescent micrographs of the macrogametocytes incubated with mouse anti-WFB pAb (visualized with Cy3, red). P Merged image of (N) and (O). Scale bars represent 10 μm

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