Fig. 2From: Examination of gametocyte protein 22 localization and oocyst wall formation in Eimeria necatrix using laser confocal microscopy and scanning electron microscopyLaser confocal microscopy (LCM) of WFBs and macrogametocytes. A–D Immunofluorescence co-staining of EnGAM22 and EnGAM59 in WFBs. A Bright-field images of the WFB-rich extract. B Fluorescent micrographs of the WFBs incubated with rabbit anti-rEnGAM22 pAb (visualized with FITC, green). C Fluorescent micrographs of the WFBs incubated with mouse anti-rEnGAM59 pAb (visualized with Cy3, red). D Merged image of (B) and (C). This shows that the enriched fractions include both intact WFB1s and WFB2s. E–H Immunofluorescence co-staining of EnGAM22 and EnGAM59 in macrogametocytes. E Bright-field images of the macrogametocytes. F Fluorescent micrographs of the macrogametocytes incubated with rabbit anti-rEnGAM22 pAb (visualized with FITC, green). G Fluorescent micrographs of the macrogametocytes incubated with mouse anti-rEnGAM59 pAb (visualized with Cy3, red). H Merged image of (F) and (G). I–L Immunofluorescence co-staining of EnGAM22 and WFB proteins in macrogametocytes. I Bright-field images of the macrogametocytes. J Fluorescent micrographs of the macrogametocytes incubated with rabbit anti-rEnGAM22 pAb (visualized with FITC, green). K Fluorescent micrographs of the macrogametocytes incubated with mouse anti-WFB pAb (visualized with Cy3, red). L Merged image of (J) and (K). M–P Immunofluorescence co-staining of EnGAM59 and WFB proteins in macrogametocytes. M Bright-field images of the macrogametocytes. N Fluorescent micrographs of the macrogametocytes incubated with rabbit anti-rEnGAM59 pAb (visualized with FITC, green). (O) Fluorescent micrographs of the macrogametocytes incubated with mouse anti-WFB pAb (visualized with Cy3, red). P Merged image of (N) and (O). Scale bars represent 10 μmBack to article page