Insecticide resistance status of Aedes aegypti in southern and northern Ghana

Parasites & Vectors

Table 1 List of primer sequences used for detecting allele-specific kdr mutations in the voltage-gated sodium channel gene of Aedes mosquitoes

kdr mutation	Primers	Sequence (5' -3')	References
V1016I	1016 Val ⁺(for)	## ACAAATTGTTTCCCACCCGCACCGG	[46]
	1016 Ile kdr (for)	#ACAAATTGTTTCCCACCCGCACTGA
	1016 common (rev)	GGATGAACCGAAATTGGACAAAAGC
F1534C	1534 Phe + (for)	#TCTACTTTGTGTTCTTCATCATATT	[46]
	1534 Cys kdr (for)	##TCTACTTTGTGTTCTTCATCATGTG
	1534 common (rev)	TCTGCTCGTTGAAGTTGTCGAT
	long 5'-tail	GCGGGCAGGGCGGCGGGGGCGGGGCC
	short 5'-tail	GCGGGC
V410L	V410fw	GAT AAT CCA AAT TAC GGG TAT AC	[47]
	V410fw [L – GC]	ATC TTC TTG GGT TCG TTC TAC CGT G
	L410fw [S – GC]	ATC TTC TTG GGT TCG TTC TAC CAT T
	410rev	ATC TTC TTG GGT TCG TTC TAC CAT T
	[L – GC]	GCG GGC AGG GCG GCG GGG GCG GGG CC
	[S – GC]	GCG GGC

⁺ wild-type specific primer, kdr kdr specific primer, ^#short 5′tail attached, ^##long 5′tail attached, fw forward primer, rev reverse primer

ISSN: 1756-3305