Fig. 4
From: Digital PCR: modern solution to parasite diagnostics and population trait genetics
Schematic representation of part of the ribosomal nuclear DNA (rDNA) gene cluster and the variability of the different loci. This example shows two DNA targets (terminal 3′ end of 5.8 S and terminal 3′ end of ITS2) used to quantify the three major strongylid nematodes in the gastrointestinal tract of sheep. The blue arrow indicates the position for which a universal primer and probe pair is used to quantify all strongylid nematode species, while the red arrow indicates the position in ITS2 where a different primer and probe pair quantifies the specific nematode genera present in a sample