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Fig. 5 | Parasites & Vectors

Fig. 5

From: Clonorchis sinensis granulin promotes malignant transformation of human intrahepatic biliary epithelial cells through interaction with M2 macrophages via regulation of STAT3  phosphorylation and the MEK/ERK pathway

Fig. 5

ae IL-6/Janus kinase 2 (JAK2)/STAT3 signaling pathway was involved in the cell–cell interaction mediated by CsGRN activated in HIBECs. a ELISA was used to determine the concentration of IL-6 in the co-culture medium. b Expression of phosphorylatedSTAT3 (p-STAT3), TFF3, BCL-2 and c-Myc in HIBECs treated with co-culture medium was detected by qPCR. C Western blot analysis of p-JAK2, p-STAT3 and c-Myc levels in the co-culture medium. d Expression of p-JAK2+ CK19+ cells and p-STAT3+ CK19+ cells in the liver of CsGRN-injected mice was assessed by IF. Scale bar 100 μM. e Western blot analysis of p-JAK2, p-STAT3 and c-Myc levels in the liver of CsGRN-treated mice. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. For other abbreviations, see Figs. 1, 2,  3 and  4

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