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Fig. 1 | Parasites & Vectors

Fig. 1

From: A rapid and visual detection assay for Clonorchis sinensis based on recombinase polymerase amplification and lateral flow dipstick

Fig. 1

Optimization of the RPA-LFD assay. A Optimization of the amplification temperature. The RPA-LFD assay results are positive at temperatures from 25 °C to 45 °C. B Optimization of the amplification duration. The PCR-LFD assay results are positive for 5, 10, 15, 20, 25 and 30 min duration. C Optimization of the amplification product dilution. The RPA-LFD assay results are positive at amplification product dilutions of 1:10, 1:20, 1:50, and 1:100

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