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Table 1 RPA-LFD primers and probe used in the present study

From: A rapid and visual detection assay for Clonorchis sinensis based on recombinase polymerase amplification and lateral flow dipstick

Primers

Length

Sequence(5′–3′)

RPA-F

32

ATATGCTTGCTGGAACTCGGGAGCGTCTATGA

RPA-R

32

TACAGAAATAACAACCGTCCTAAACGACCCTA

Lateral flow reverse primer

32

Biotin-TACAGAAATAACAACCGTCCTAAACGACCCTA

Lateral flow probe

46

FAM-CTTTCTGCTTCTGTAATTGATGCCTTGTTT-THF-CATGATACTTGGTTTG-C3 Spacer

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Parasites & Vectors

ISSN: 1756-3305

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