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Fig. 2 | Parasites & Vectors

Fig. 2

From: Co-infection of dengue and Zika viruses mutually enhances viral replication in the mosquito Aedes aegypti

Fig. 2

Sequential co-infection modulates viral NS1 subcellular localization and viral replication in mosquito cells. A Time course of sequential infection with ZIKV and DENV2. B Subcellular localization of flaviviral NS1 (green) and ZIKV-E protein (red) in single-infected and ZIKV → DENV2 sequentially co-infected cells at 2 dpi, with DAPI (blue)-stained DNA demarcating nuclei. Scale bars, 10 μm. C Relative levels (2−(dCt)) of DENV and ZIKV viral genomes in secreted viruses in the supernatant of cell culture medium at 2 dpi. These results are representative of at least three independent experiments. D At 2 dpi, culture supernatants from single-infected and ZIKV → DENV2 sequentially co-infected cells were collected and used in a focus-forming assay. NS1 (green) stained for replicating virus

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