Fig. 5

Virus production and vector susceptibility are enhanced in DENV2/ZIKV co-infected mosquitos. A Mosquitos were challenged with virus as described in Fig. 4, and then collected at 7 dpi (day 12) for plaque assay. Geometric means (PFU/ml) are plotted, and each of eight viral challenge schemes comprised at least three biological cohorts. B Sample size n, infection rate, and median PFU/ml corresponding to the experimental groups. Infected samples had positive (> 0) PFU/ml values; uninfected, negative samples are represented on the log scale as positive (PFU/ml = 1) only for visual interpretation. Comparison between groups post hoc was performed using Dunn’s multiple comparisons test; *P < 0.05; **P < 0.01; ***P < 0.001