Honokiol induces apoptosis-like death in Cryptocaryon irritans Tomont

Background Cryptocaryon irritans, a common parasite in tropical and subtropical marine teleost fish, has caused serious harm to the marine aquaculture industry. Honokiol was proven to induce C. irritans tomont cytoplasm shrinkage and death in our previous study, but the mechanism by which it works remains unknown. Methods In this study, the changes of apoptotic morphology and apoptotic ratio were detected by microscopic observation and AnnexinV-FITC/PI staining. The effects of honokiol on intracellular calcium ([Ca2+]i) concentration, mitochondrial membrane potential (ΔΨm), reactive oxygen species (ROS), quantity of DNA fragmentations (QDF) and caspase activities were detected by Fluo-3 staining, JC-1 staining, DCFH-DA staining, Tunel method and caspase activity assay kit. The effects of honokiol on mRNA expression levels of 61 apoptosis-related genes in tomonts of C. irritans were detected by real-time PCR. Results The results of the study on the effects of honokiol concentration on C. irritans tomont apoptosis-like death showed that the highest levels of prophase apoptosis-like death rate (PADR), [Ca2+]i concentration, ROS, the activities of caspase-3/9 and the lowest necrosis ratio (NER) were obtained at a concentration of 1 μg/ml, which was considered the most suitable for inducing C. irritans tomont apoptosis-like death. When C. irritans tomonts were treated with 1 μg/ml honokiol, the [Ca2+]i concentration began to increase significantly at 1 h. Following this, the ROS, QDF and activities of caspase-3/9 began to increase significantly, and the ΔΨm began to decrease significantly at 2 h; the highest PADR was obtained at 4 h. The mRNA expression of 14 genes was significantly upregulated during honokiol treatment. Of these genes, itpr2, capn1, mc, actg1, actb, parp2, traf2 and fos were enriched in the pathway related to apoptosis induced by endoplasmic reticulum (ER) stress. Conclusions This article shows that honokiol can induce C. irritans tomont apoptosis-like death. These results suggest that honokiol may disrupt [Ca2+]i homeostasis in ER and then induce C. irritans tomont apoptosis-like death by caspase cascade or mitochondrial pathway, which might represent a novel therapeutic intervention for C. irritans infection. Graphical abstract Supplementary Information The online version contains supplementary material available at 10.1186/s13071-023-05910-1.


Background
Cryptocaryon irritans, a common protozoan parasite of marine teleost fish, causes "white spot" disease [1].This disease is mainly prevalent in tropical and subtropical sea areas [2][3][4].Its life cycle includes four stages: trophont, protomont, tomont, and theront [5].Tomont is the longest-lasting, free-living stage of C. irritans.Tomonts have strong resistance to drugs and harsh environments because of their hard cysts.Tomonts can still produce infectious theronts after being preserved at 12 °C for 3 months [5].It is difficult to completely remove C. irritans tomonts in an open mariculture environment, which makes the prevention and control of white spot disease very difficult.It is a good strategy to prevent and treat parasites by inducing their spontaneous death; this has the advantages of low probability of drug resistance and host inflammation.It is well known that apoptosis is a highly regulated process of cell death [6].In recent years, apoptosis has provided a new treatment for many diseases, such as inflammation, cancer, leishmaniasis, malaria, and toxoplasmosis [7][8][9][10][11][12][13][14].The apoptosis-like death pathway has also been found in many protozoa, such as Leishmania, Plasmodium falciparum, Tetrahymena thermophila, Trypanosoma cruzi, Blastocystis hominis, Toxoplasma gondii, and Ichthyophthirius multifiliis [12][13][14][15][16][17][18][19][20][21], providing a new way to treat parasitic diseases.Many apoptosis-related genes of C. irritans have been found via transcriptome analysis [22][23][24][25][26], which indicates that C. irritans might have an apoptosis-like death pathway.Honokiol, one of the main active components of Magnolia officinalis, has been reported to induce apoptosis of cancer cells and Candida albicans via the endoplasmic reticulum (ER) stress pathway [27][28][29][30].Our previous studies demonstrated that honokiol significantly inhibited the proliferation and hatching of C. irritans tomonts.The resulting C. irritans tomont cytoplasm obviously shrank without cytoplasm or cell membrane damage [31], indicating that honokiol might induce C. irritans tomont apoptosis-like death.However, further experiments are needed to confirm this speculation, and the mechanism remains to be uncovered.
In this article, Annexin V-FITC/PI staining was used to determine whether honokiol induces C. irritans tomont apoptosis-like death.The effects of honokiol at various treating concentrations and times on the morphologies, normal rate (NOR), prophase apoptosis-like death rate (PADR), anaphase apoptosis-like death rate (AADR), necrosis rate (NER), intracellular calcium concentration ([Ca 2+ ] i concentration), mitochondrial membrane potential (ΔΨm), reactive oxygen species (ROS), quantity of DNA fragmentations (QDF), caspase activities, and mRNA expression of apoptosis-related genes of C. irritans tomont were investigated to uncover the mechanism of honokiol for inducing C. irritans tomont apoptosislike death.
Cryptocaryon irritans used in this study were isolated from T. ovatus with white spot disease, which were obtained from a marine cage culture in Lingao County, Hainan Province, P.R. China, and passaged and propagated in T. ovatus [31].All C. irritans tomonts used in this study were newly collected between 8:00 a.m. and 9:00 a.m. on the day of the experiment.

Experiment design
Cryptocaryon irritans tomonts were collected, resuspended in 1 ml filter-sterilized seawater, and added to the assigned wells in a 24-well microplate.Then, 10 μl of 0 (control sample), 60, 100, 200, 400, and 800 μg/ ml honokiol 50% (v/v) ethanol aqueous solutions were added to the assigned wells in the 24-well microplates, respectively.The final honokiol concentrations were 0.0 (control sample), 0.6, 1.0, 2.0, 4.0, and 8.0 μg/ml, respectively.The 24-well microplates were placed in a light incubator (GZX250E, Tianjin Taisite Instrument Co., Ltd., P.R. China) at 27 ± 0.5 °C for 8 h.The morphologies, NOR, PADR, AADR, NER, [Ca 2+ ] i concentration, ΔΨm, ROS, QDF, and caspase activities of the above-treated C. irritans tomonts were analyzed using a direct microscopic observation method, an Annexin V-FITC/PI apoptosis detection kit, a Fluo-3 AM calcium concentration detection kit, a mitochondrial membrane potential assay kit, a reactive oxygen species assay kit, a terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) apoptosis assay kit, and a caspase-3/8/9 activity assay kit, respectively.Cryptocaryon irritans tomonts were treated with honokiol at the optimum concentration according to the results of the above experiments for 0, 1, 2, 4, 8, and 16 h.Their morphologies, NOR, PADR, AADR, NER, [Ca 2+ ] i concentration, ΔΨm, ROS, QDF, and caspase activities were also analyzed using the same methods as mentioned above.Cryptocaryon irritans tomonts were treated with honokiol at the conditions with the highest apoptosis rate of tomonts according to the results of the above experiments, and then the inhibition rate of tomonts hatching was observed according to the method described by Zhong et al. [31].Each well assigned to caspase activity analysis and the mRNA expression of apoptosis-related genes analysis contained 1000 C. irritans tomonts, while each well assigned to the other analyses contained 100 C. irritans tomonts.Each analysis was carried out five times in parallel.

Morphology observation and Annexin V-FITC/PI stain
The NOR, PADR, AADR, and NER of C. irritans tomonts were analyzed using an Annexin V-FITC/PI apoptosis detection kit (Beijing Solarbio Science & Technology Co., Ltd., P.R. China).The C. irritans tomonts, treated as described in "Experiment Design, " were washed twice with 1 ml ice-cold PBS (pH = 7.4, 0.01 mol/l) and resuspended in 100 μl binding buffer.A solution of 5 μl Annexin V-FITC was added, and the tomonts were incubated at 27 ± 0.5 ℃ in the dark for 10 min.Then, a solution of 5 μl PI was added, and the tomonts were incubated at 27 ± 0.5 ℃ in the dark for 5 min, washed twice with 1 ml ice-cold PBS, and transferred to 384-well microplates (each well contained a C. irritans tomont).A total of 150 C. irritans tomonts were analyzed in parallel with a fluorescence microplate reader (BioTek Synergy H1, BioTek Instruments, Inc., USA) at Ex/Em = 488/525 nm and Ex/ Em = 488/630 nm, and their micromorphologies were observed under a fluorescence inversion microscope (DMi8 + DFC7000T, Leica Microsystems, Germany).Four-quadrant apoptosis diagrams were drawn using a logarithm transformed Annexin V-FITC fluorescence intensity [LG (A Ex/Em = 488/525 nm )] as the abscissa axis and a logarithm transformed PI fluorescence intensity [LG (A Ex/Em = 488/630 nm )] as the ordinate axis.Normal (in the I quadrant), prophase apoptosis-like death (in the II quadrant), anaphase apoptosis-like death (in the III quadrant), and necrosis (in the IV quadrant) C. irritans tomonts were identified according to the four-quadrant apoptosis diagrams, and their numbers were recorded [28].Finally, the percentages of the NOR, PADR, AADR, NER, and C. irritans tomonts were calculated.

Caspase activity determination
The activities of caspases, including caspase-3/8/9 in the C. irritans tomonts, were respectively determined using a caspase-3/8/9 activity assay kit (Beijing Solarbio Science & Technology Co., Ltd., P.R. China).On ice, the C. irritans tomonts, treated as described in "Experiment Design, " were washed twice in 1 ml ice-cold PBS (pH = 7.4, 0.01 mol/l), a lysis buffer was added at a ratio of 1: 10 (C.irritans tomonts mass: lysis buffer, g:ml), ground to homogenate on the ice using a grinding rod, cracked in ice for 15 min, and centrifugated (15000 g, 4 ℃, 15 min).The supernatants were then collected.The supernatants, caspase reaction buffer, and substrates (Asp-Glu-Val-Asp-p-nitroanilide for caspase-3 activity determination, N-acetyl-Ile-Glu-Thr-Asp-p-nitroanilide for caspase-8 activity determination, and Leu-Glu-His-Asp-p-nitroanilide for caspase-9 activity determination) were added to 96-well enzyme plates and incubated at 37 ℃ for 8 h, determined by microplate reader (BioTek Synergy H1, BioTek Instruments, Inc., USA) at 405 nm.The activities of caspase-3/8/9 were calculated using the following function: where X is the result of the calculation of the absorbance at 405 nm in the caspase standard curve (μmol/l), V 1 is the total volume of the reaction system (μl), V 2 is the volume of the added supernatant (μl), T is the reaction time (h), and C protein is the total protein concentration in the supernatant (mg/ml) determined by the Bradford method [33].

Effects of honokiol on the mRNA expression of apoptosis-related genes
The C. irritans tomonts were treated with honokiol at the optimum concentration obtained according to the results of experiment design for 0, 1, 2, 4, 8, and 16 h, and the mRNA expression of the 61 apoptosis-related genes obtained by blast according to the C. irritans genome (SRX12890364, SRX12890363) [34] was analyzed using the RT-PCR method.Each well assigned to mRNA expression of apoptosis-related gene analysis contained 1000 C. irritans tomonts.Each analysis was carried out in triplicate.The 61 apoptosis-related genes and their primers designed by the Primer 3 plus software (https:// www.prime r3plus.com/) according to the primer design rules [70] are listed in Additional file 1: Table S1.The specificities of the primers used in this study were confirmed using BLAST tool (https:// blast.ncbi.nlm.nih.gov/ Blast.cgi).Eastep Super Total RNA Extraction LS1040 (Promega Corp., USA) was used according to the manufacturer's instructions to extract total RNA by the glass tissue homogenizer from the treated C. irritans tomonts, and DNase I (Promega Corp., USA) was used to digest the contaminating DNA.Then, agarose gel electrophoresis and an ultramicro spectrophotometer (NanoPhotometer NP80, IMPLEN GMBH, Germany) were used to ensure the quality of RNA, and the cDNA sequence was synthesized using Eastep RT Master MIX Kit LS2054 (Promega Corp., USA).The mRNA expressions were detected using the CFX96 Real-Time PCR Detection System (Bio-Rad, Hercules, CA, USA) with a 10 μl reaction mixture containing 5 μl (2 ×) Eastep qPCR Master Mix (Vazyme Biotech Co., Ltd., P.R. China), 1.0 μl cDNA template, 0.2 μl each primer (10 μmol/l), and 3.6 μl nuclease-free water.Each sample was tested in triplicate.The real-time PCR cycling conditions were as follows: 120 s 95 °C initial denaturation, 40 cycles of 15 s 95 °C denaturation, and 60 s 60 °C annealing/extension.Dissociation curve analysis (65 to 95 °C: increment 0.5 °C for 5 s) was performed to verify the amplification of a single product.The C. irritans 18S rRNA gene sequence (JN636814.1)was used as the internal reference gene.Furthermore, the specificities of the primers were confirmed by the fluorescence quantitative melting curve used the CFX96 Real-Time PCR Detection System (Bio-Rad, Hercules, CA, USA).After real-time PCR, the mRNA expression of the apoptosisrelated genes was calculated u the method of 2 −△△Ct .

Statistical analysis
All the experimental indexes were tested using Tukey's test with SPSS 25.0 (IBM Corp., USA) to determine the significant difference between the experimental sample and the control sample.A difference was considered significant when the p values were < 0.05 and extremely significant when the p values were < 0.01.The chart was plotted using OriginPro 9.0 (OriginLab Corp., USA), and the data were expressed as mean ± standard deviation (SD).

Results of morphology observation and Annexin V-FITC/PI stain
The morphologies of the unstained and AnnexinV-FITC/ PI-stained C. irritans tomonts treated with honokiol are given in Fig. 1, showing that all the honokiol-treated C. irritans tomonts' cytoplasms obviously shrank, their cell membranes were separated from cysts, and they were stained with Annexin V-FITC (showing green fluorescence) in a dose-dependent manner; this indicates phosphatidylserine valgus in cell membrane (a typical cell apoptosis characteristic).When the concentration of honokiol was > 4.0 μg/ml, the treated C. irritans tomonts' cytoplasms were irregularly condensed, became hyaline, and were stained by PI (showing red fluorescence), which indicates their cell membranes were damaged (a typical characteristic of middle-and late-stage cell apoptosis or necrosis).Four-quadrant apoptosis diagrams are given in Fig. 2, showing that with the increase in honokiol, the PADR began to increase at a concentration of 0.6 μg/ml.It reached its highest level when the honokiol concentration was 1.0 μg/ml and then decreased, while the AADR and NER began to increase at a concentration of 2.0 μg/ ml.

Effect of honokiol on [Ca 2+
] i concentration, ΔΨm, ROS, QDF, and caspase-3/8/9 activities The [Ca 2+ ] i concentration and the ΔΨm, ROS, QDF, and caspase-3/8/9 activities in the C. irritans tomonts treated with honokiol at various concentrations are given in Fig. 3.As shown in Fig. 3A, with the increase in the honokiol concentration, the [Ca 2+ ] i concentration increased to a level significantly higher than that of the control sample at 0.6 μg/ml.It reached the highest level at 1 μg/ml, returned to the level of the control sample Fig. 1 Morphologies of honokiol-treated Cryptocaryon irritans tomonts stained and unstained with Annexin V-FITC/PI.The C. irritans tomonts were treated with honokiol at 0.0, 0.6, 1.0, 2.0, 4.0, and 8.0 μg/ml for 8 h.The treated tomonts were incubated with Annexin V-FITC and a PI probe and observed under a fluorescence inversion microscope (DMi8 + DFC7000T, Leica Microsystems, Germany).The results show that all the honokiol-treated C. irritans tomonts' cytoplasms obviously shrank and were stained with Annexin V-FITC.When the concentration of honokiol was > 4.0 μg/ml, the treated C. irritans tomonts' cytoplasms were irregularly condensed, became hyaline, and were stained by PI. a-f: Morphologies of C. irritans tomonts respectively treated with 0.0, 0.6, 1.0, 2.0, 4.0, and 8.0 μg/ml honokiol for 8 h.g-l: Morphologies of C. irritans tomonts respectively treated with 0.0, 0.6, 1.0, 2.0, 4.0, and 8.0 μg/ml honokiol for 8 h and observed at Ex/Em = 488/525 nm.m-r: Morphologies of C. irritans tomonts respectively treated with 0.0, 0.6, 1.0, 2.0, 4.0, and 8.0 μg/ml honokiol for 8 h and observed at Ex/Em = 488/630 nm.s-x: Overlapping morphology photos of C. irritans tomonts respectively treated with 0.0, 0.6, 1.0, 2.0, 4.0, and 8.0 μg/ml honokiol for 8 h and recorded at Ex/ Em = 488/525 nm and Ex/Em = 488/630 nm.All bars = 300 μm at 2.0 μg/ml, and then decreased to a level significantly lower than that of the control sample when the honokiol concentration increased > 4.0 μg/ml.As shown in Fig. 3B, the ΔΨm decreased to a level significantly lower than that of the control sample when the honokiol concentration was > 0.6 μg/ml.As shown in Fig. 3C, with the increase of the honokiol concentration, the ROS increased to a level significantly higher than that of the control sample at 1.0 μg/ml and then returned to the level of the control sample.As Fig. 3D shows, with the increase of the honokiol concentration, the QDF began to increase at 0.6 μg/ml, increased to a level significantly higher than that of the control sample at 1.0 μg/ml, reached the highest level at 2.0 μg/ml, and then decreased, but the level remained significantly higher than that of the control sample when the honokiol concentration increased above 4.0 μg/ml.As shown in Fig. 3E, with the increase of the honokiol concentration, both the caspase-3/9 activities began to increase to levels significantly higher than those of the control sample at 0.6 μg/ml and reached the highest levels at 1.0 μg/ml.The activity of caspase-3 gradually returned to the level of the control sample when the honokiol concentration was ≥ 4.0 μg/ml, while the activity of caspase-9 remained at a level higher than that of the control sample, and the activity of caspase-8 always remained at the level of the control sample.

Effects of honokiol treatment time on C. irritans tomont apoptosis-like death
The results found in "Effect of honokiol on [Ca 2+ ] i concentration, ΔΨm, ROS, QDF, and caspase-3/8/9 activities" showed that C. irritans tomonts had the highest PADR, [Ca 2+ ] i concentration, ROS, caspase-3/9 activities, and higher QDF, and the lowest NER when the honokiol concentration was 1.0 μg/ml.Therefore, 1.0 μg/ml was considered the optimum honokiol concentration for inducing C. irritans tomont apoptosislike death.Together with the extension of the treatment time, the changes in the NOR, PADR, AADR, NER, [Ca 2+ ] i concentration, ΔΨm, ROS, QDF, and the caspase-3/8/9 activities for the C. irritans tomonts are given in Fig. 4, showing that, with the extension of the treatment time, the [Ca 2+ ] i concentration and PADR began to increase significantly at 1 h, followed by ROS and QDF.The caspase-3/9 activities began to increase significantly, and the ΔΨm began to decrease significantly at 2 h.The [Ca 2+ ] i concentration, PADR, ROS, QDF, and caspase-3/9 activities reached the highest Fig. 2 Four-quadrant apoptosis diagrams of Cryptocaryon irritans tomonts treated with honokiol at various concentrations for 8 h.The C. irritans tomonts were treated with honokiol at 0.0, 0.6, 1.0, 2.0, 4.0, and 8.0 μg/ml for 8 h.The treated tomonts were incubated with Annexin V-FITC and a PI probe and analyzed with a fluorescence microplate reader (BioTek Synergy H1, BioTek Instruments, Inc., USA).The results show that with the increase in honokiol concentration, the PADR began to increase at 0.6 μg/ml and reached the highest level at 1.0 μg/ml, while the AADR and NER began to increase at 2.0 μg/ml levels at 4 h and then slightly decreased but remained at levels significantly higher than those at 0 h, while the ΔΨm continued to decrease at 16 h, and the AADR began to significantly increase at 8 h.The NER and the activity of caspase-8 showed nonsignificant changes at 16 h.These results suggest that the optimum treatment time for 1.0 μg/ml honokiol inducing C. irritans tomonts apoptosis-like death is 4 h.The inhibition rate The result shows that the [Ca 2+ ] i concentration began to increase significantly at 1 h, and then the ROS, QDF, and caspase-3/9 activities began to increase significantly and the ΔΨm began to decrease significantly at 2 h; the highest PADR was obtained at 4 h.The results are expressed as mean ± SD, n = 5. *Significant difference from the control sample (0.0 μg/ml), P < 0.05.**Highly significant difference from the control sample (0.0 μg/ml), P < 0.01 (See figure on next page.)Fig. 4 (See legend on previous page.) of tomont hatching was 52.38% after treatment with 1.0 μg/ml honokiol for 4 h.

Effects of honokiol on the mRNA expression of apoptosis-related genes
Among the 61 investigated apoptosis-related genes, 14 were significantly upregulated (shown in Fig. 5).The fluorescence quantitative melting curves of the 14 genes are shown in Additional file 2: Fig. S1.Among the 14 upregulated genes, itpr2, capn1, mc, actg1, actb, parp2, traf2, and fos were enriched in the pathway related to apoptosis induced by the disruption of the [Ca 2+ ] homeostasis in ER.Among the eight genes, fos was significantly upregulated at 4 h, while the other genes were significantly upregulated within 2 h.Gene gzmb, enriched in the Granzyme B pathway, was significantly upregulated at 16 h, and gene tuba1c, enriched downstream of the Granzyme B pathway, was significantly upregulated at 2 h.Genes hras and raf1, enriched in the MAPK signaling pathway, were significantly upregulated at 2 h, and gene hras was also significantly upregulated at 4 h.Gene akt1, enriched in the PI3K-Akt signaling pathway, was significantly upregulated at 2 h.Gene atm, the upstream regulatory of the p53 signaling pathway, was significantly upregulated at 2, 4, and 16 h.

Discussion
The apoptosis-like death pathway has been found in many protozoa, such as Leishmania, P. falciparum, T. thermophila, T. cruzi, B. hominis, T. gondii, and I. multifiliis [12][13][14][15][16][17][18][19][20][21].Ichthyophthirius multifiliis is the pathogen of freshwater white spot disease, the morphology and life cycle of which are similar to those of C. irritans [1].It has been reported that fish skin antibodies could cause I. multifiliis apoptosis-like phenomena, such as PS externalization and chromatin condensation [16].It has also been reported that malachite green could cause I. multifiliis apoptosis-like phenomena, such as mitochondrial swelling, mitochondrial membrane integrity destruction, ribosome number change, and PS externalization through the PI3K-Akt signal pathway [21].In this study, honokiol was demonstrated to cause significant C. irritans tomont cytoplasm atrophy, cell volume reduction, PS externalization, a significant increase in QDF, [Ca 2+ ] i concentration, ROS and caspase-3/9 activities, a significant decrease in ΔΨm, and significant upregulation of the mRNA expression of the 14 apoptosis-related genes; this strongly suggests that C. irritans tomonts have a form of regulated apoptosis-like death.The apoptosis-like death named by the Nomenclature Committee on Cell Death (NCCD) resembles the apoptosis of metazoans [35] and has been considered an ideal strategy to prevent and treat parasitic diseases [11,36].Although this study has proved that apoptosis-like death exists in C. irritans tomonts, which provides a potential new way to treat marine fish white spot disease with the advantages of a lower probability of drug resistance and adverse effects, further studies are needed to uncover and confirm the mechanism of honokiol inducing C. irritans tomont apoptosis-like death.
Honokiol, as one of the main active components of M. officinalis, has been reported to induce apoptosis of several cells, such as neuroblastoma cells, A549 cells, 95-D cells, and human chondrosarcoma cells, fungi such as C. albicans, and protozoan parasites such as Leishmania via the ER stress pathway [8,[27][28][29][30][36][37][38][39].The reported ER stress pathway inducing cell apoptosis is summarized in Fig. 6, which shows that drugs such as honokiol and physiological or environmental factors can cause excessive or aberrant ER stress [30,[40][41][42].Excessive or aberrant ER stress leads to Ca 2+ release from ER via the inositol 1,4,5-trisphosphate receptor (IP3Rs) and to unfolded protein response (UPR) accumulation [43][44][45][46][47]. Cho et al. [48] reported that honokiol could promote [Ca 2+ ] i release and increase [Ca 2+ ] i concentration by inhibiting the activity of endoplasmic reticulum protein 44 (ERP44), which has inhibitory activity on the calcium channel IP3Rs [49].Similarly, the [Ca 2+ ] i concentration first significantly increased in C. irritans tomonts in this Fig. 5 mRNA expression of apoptosis-related genes in Cryptocaryon irritans tomonts treated with honokiol.The mRNA expression of the apoptosis-related genes in C. irritans tomonts were respectively treated with 1.0 μg/ml honokiol at 0, 1, 2, 4, 8, and 16 h.The results show that a total of 14 genes increased significantly at different times.The results are expressed as mean ± SD, n = 3. *Significant difference from the control (0.0 μg/ml), P < 0.05.**Highly significant difference from the control (0.0 μg/ml), P < 0.01 Fig. 6 Regulatory mechanism of apoptosis induced by ER stress.This figure shows that the classic ER stress pathway induces cell apoptosis.Apaf1, Cytc, Fodrin, PERK, eiF2α, IRE1α, TRAF2, ASK1, and API were coded with apaf1, cycs, sptan1, eif2ak3, eif2s1, ern1, traf2, map3k5, and fos, respectively.Beta-actin and γ-actin were respectively coded with actb and actg1.Calpains included the calpain-1 catalytic subunit and the calpain-2 catalytic subunit, which were coded with capn1 and capn2.PARPs include poly [ADP-ribose] polymerase 2 and poly [ADP-ribose] polymerase 4, which were coded with parp2 and parp4.JNKs include mitogen-activated protein kinase 9 and mitogen-activated protein kinase 10, which were respectively coded with mapk9 and mapk10.IP3Rs include inositol 1,4,5-trisphosphate receptor type 2 and inositol 1,4,5-trisphosphate receptor type 3, which were respectively coded with itpr1, itpr2, and itpr3.The mRNA expression of itpr2, capn1, mc, actg1, actb, parp2, traf2, and fos increased significantly in this study study.The increase of Ca 2+ can cause two significant reactions, Reaction I and Reaction II.In Reaction I, the increase of Ca 2+ can provoke the cascade reaction of caspases, including calpains, caspase-12, and caspase-3 [50][51][52].Coinciding with Reaction I, the mRNA expression of gene capn1 coding calpain was significantly upregulated and the activity of caspase-3 significantly increased in C. irritans tomonts.Via blasting in the C. irritans genome, gene caspase-12 coding caspase-12 was not found, but gene mc coding metacaspase was found, and its mRNA expression was also significantly upregulated.Metacaspase has been reported to have an apoptosis regulation function in protozoa such as Leishmania and Plasmodium, which is similar to caspase-12 in metazoa [11,53,54].In Reaction II, the increase of [Ca 2+ ] i can also decrease the ΔΨm of mitochondria and then promote ROS production and release from mitochondria [44,55,56].An increase in ROS can further promote [Ca 2+ ] I release and increase QDF, and then the mitochondria sequentially activate caspase-9 and caspase-3 via apoptotic protease-activating factor 1 (Apaf1) combined with cytochrome c (CytC) [45,57].In agreement with Reaction II, honokiol has been proven to significantly decrease ΔΨm of mitochondria and increase ROS production, the activities of caspase-9 and caspase-3, and QDF in C. irritans tomonts.The activated caspase-3 can hydrolyze and deactivate actins and poly ADP-ribose polymerase (PARPs) [58][59][60].Actins are involved in the maintenance of the cytoskeleton.Therefore, hydrolyzed actins cause cytoplasm atrophy and cell volume reduction [61][62][63], while hydrolyzed PARPs cause irreversible DNA damage and QDF increase [64][65][66].In supporting these phenomena, this article has proven that honokiol could significantly upregulate the mRNA expressions of genes actg1, actb, and parp2, which respectively code actins and PARPs, cause C. irritans tomont cytoplasm atrophy and cell volume reduction, and increase the QDF [67][68][69].Besides this apoptosis pathway, caused by ER stress via Ca 2+ , the ER stress also leads to the accumulation of UPR.Under homeostatic conditions, proteins involved in the UPR, protein kinase RNA-like endoplasmic reticulum kinase (PERK), activating transcription factor 6 (ATF6), and inositol-requiring enzyme 1α (IRE1α) are bound to glucose-regulated protein 78 (GRP78) by their ER lumen domains, keeping them inactive.With the accumulation of UPR, causing GRP78 to leave PERK, ATF6, and IRE1α, the activation of these proteins is induced [45][46][47] (Fig. 6).The activated PERK activates the cascade reaction, including eukaryotic translation initiation factor 2 subunit alpha (eiF2α), activating transcription factor 4 (ATF4), and DNA damage inducible transcript 3 (DDIT3).DDIT3 can decrease the expression of antiapoptosis proteins such as B-cell lymphoma 2 (Bcl-2) and increase the expression of pro-apoptosis proteins such as Bcl-2 interacting mediator of cell death (Bim).Then, the mitochondria sequentially activate caspase-9 and caspase-3 via Apaf1 combined with CytC.Finally, cell apoptosis is induced [46].The activated ATF6 also activates DDIT3 and induces apoptosis via the mitochondria pathway.The activated IRE1α, combined with TNF receptorassociated factor 2 (TRAF2), activates cascade reactions, including apoptosis signal-regulating kinase 1 (ASK1) and c-jun n-terminal kinase (JNKs).JNKs are responsible for phosphorylation of the mitochondrial proteins Bim (pro-apoptosis) and Bcl2 (anti-apoptosis), which are activated and inhibited, respectively.Then, the activated JNKs induce the mitochondria apoptosis pathway or upregulate the expression of the jun proto-oncogene (c-jun) and fos proto-oncogene (API), increase the QDF, and induce cell apoptosis [45][46][47].Although the mRNA expressions of traf2 and fos in this pathway were significantly upregulated by honokiol, those of eif2ak3, eif2s1, ern1, map3k5, mapk9, and mapk10 showed nonsignificant changes when C. irritans tomonts were treated with honokiol.This suggests that honokiol might not induce C. irritans tomont apoptosis via this pathway.In summary, the results of this study suggest that honokiol might inhibit the activity of ERP44 and unlimited IP3R release [Ca 2+ ] i , disrupt [Ca 2+ ] i homeostasis in ER, and then induce C. irritans tomont apoptosis-like death by caspase cascade or mitochondrial pathway.However, further studies such as the transcriptomic or proteomic analyses are needed to confirm this suggestion.

Conclusion
This article showed that honokiol can induce C. irritans tomont apoptosis-like death and suggested that honokiol may disrupt [Ca 2+ ] i homeostasis in ER and then induce C. irritans tomont apoptosis-like death by caspase cascade and mitochondrial pathway.This might represent a novel therapeutic intervention for C. irritans infection.Next, to further research on safe and efficient anti-C.irritans drugs, the intracellular target of honokiol in C. irritans needs to be verified.