Molecular detection of Leishmania donovani, Leishmania major, and Trypanosoma species in Sergentomyia squamipleuris sand flies from a visceral leishmaniasis focus in Merti sub-County, eastern Kenya

Background Visceral leishmaniasis (VL) and zoonotic cutaneous leishmaniasis (ZCL) are of public health concern in Merti sub-County, Kenya, but epidemiological data on transmission, vector abundance, distribution, and reservoir hosts remain limited. To better understand the disease and inform control measures to reduce transmission, we investigated the abundance and distribution of sand fly species responsible for Leishmania transmission in the sub-County and their blood-meal hosts. Methods We conducted an entomological survey in five villages with reported cases of VL in Merti sub-County, Kenya, using CDC miniature light traps and castor oil sticky papers. Sand flies were dissected and identified to the species level using standard taxonomic keys and PCR analysis of the cytochrome c oxidase subunit 1 (cox1) gene. Leishmania parasites were detected and identified by PCR and sequencing of internal transcribed spacer 1 (ITS1) genes. Blood-meal sources of engorged females were identified by high-resolution melting analysis of vertebrate cytochrome b (cyt-b) gene PCR products. Results We sampled 526 sand flies consisting of 8 species, Phlebotomus orientalis (1.52%; n = 8), and 7 Sergentomyia spp. Sergentomyia squamipleuris was the most abundant sand fly species (78.71%; n = 414) followed by Sergentomyia clydei (10.46%; n = 55). Leishmania major, Leishmania donovani, and Trypanosoma DNA were detected in S. squamipleuris specimens. Humans were the main sources of sand fly blood meals. However, we also detected mixed blood meals; one S. squamipleuris specimen had fed on both human and mouse (Mus musculus) blood, while two Ph. orientalis specimens fed on human, hyrax (Procavia capensis), and mouse (Mus musculus) blood. Conclusions Our findings implicate the potential involvement of S. squamipleuris in the transmission of Leishmania and question the dogma that human leishmaniases in the Old World are exclusively transmitted by sand flies of the Phlebotomus genus. The presence of Trypanosoma spp. may indicate mechanical transmission, whose efficiency should be investigated. Host preference analysis revealed the possibility of zoonotic transmission of leishmaniasis and other pathogens in the sub-County. Leishmania major and L. donovani are known to cause ZCL and VL, respectively. However, the reservoir status of the parasites is not uniform. Further studies are needed to determine the reservoir hosts of Leishmania spp. in the area.


Introduction
Cutaneous leishmaniasis (CL) and visceral leishmaniases (VL) are vector-borne parasitic diseases that are caused by protozoan parasites of the Leishmania genus and transmitted by infected female sand flies during a blood meal [1]. Cutaneous leishmaniasis and VL are among the world's most neglected tropical diseases (NTDs), occurring mainly in remote foci of the tropical, subtropical, and Mediterranean regions. Approximately 350 million people are at risk of infection [2]. Cutaneous leishmaniasis is the most common form with over 600,000 annual cases worldwide [3] and contributes to high psychological morbidities because of its scarring and stigmatising lesions [4]. In contrast, VL is the most fatal form of leishmaniasis with a fatality rate of nearly 100%, which occurs within 2 years if left untreated [3].
In Kenya, both VL and CL are endemic in arid and semi-arid areas in the Rift Valley, eastern and northeastern regions in the country [5]. Cutaneous leishmaniasis is caused mainly by three species of Leishmania: Leishmania tropica, Leishmania major, and Leishmania aethiopica [2,6,7]. Cutaneous leishmaniasis infections caused by L. aethiopica and L. tropica are predominant in highland areas, especially around the Mount Elgon and Rift Valley regions, respectively [7]. In contrast, L. major infections and VL, caused by L. donovani, are common in low altitude arid and semi-arid areas of the Rift Valley, eastern and north-eastern Kenya [5,8].
Sand flies of the Phlebotomus genus are the confirmed vectors of human leishmaniasis in the Old World [1]. In Kenya, the sand flies Phlebotomus guggisbergi, Phlebotomus duboscqi, and Phlebotomus pedifer are the confirmed vectors of L. tropica, L. major, and L. aethiopica, respectively [2,9]. In contrast, L. donovani is transmitted primarily by Phlebotomus martini, while Phlebotomus orientalis is regarded as a secondary vector of the parasite in Kenya [9,10]. Sand fly species belonging to the Sergentomyia genus are regarded as non-vectors of human leishmaniases since some species within this genus do not support the development of Leishmania parasites within their midgut [11,12]. However, Sergentomyia sand flies are the predominant species in most leishmaniasis endemic foci in the Old World, where they appear to be well adapted to different biotopes and environmental conditions [13]. Most of these species have been shown to feed on humans and other mammalian hosts [2] which may carry other pathogens in addition to Leishmania species.
Various studies have recently suggested the possible role of some Sergentomyia species in the transmission of Leishmania parasites pathogenic to humans. For instance, studies conducted in CL hotspots in other countries revealed the presence of L. major DNA in Sergentomyia sintoni, Sergentomyia darlingi, and Sergentomyia minuta [13][14][15][16]. Furthermore, Mukherjee and colleagues identified L. donovani infections in Sergentomyia babu in India [17]. Other reports from a CL outbreak area in Ghana showed that L. major, L. tropica, and Trypanosoma spp. can infect Sergentomyia ingrami, Sergentomyia hamoni, and Sergentomyia africana africana sand flies, respectively [18]. Live L. major promastigotes have also been isolated from Sergentomyia garnhami in Kenya [19].
Knowledge of vectors, feeding preference, and the Leishmania species in circulation are crucial for the control of leishmaniasis transmission in diseaseendemic areas. However, these data are scarce for Merti sub-County where repeated outbreaks of leishmaniasis have been reported. We designed this study to identify the sand fly species with the potential to transmit Leishmania parasites in Merti sub-County, Kenya, as well as determining their abundance, distribution, and blood-meal sources. The information would contribute to a better understanding of the disease and the implementation of targeted vector control measures.

Study area
We conducted an entomological survey in July 2018 in five villages in Merti sub-County ( Fig. 1 [20]. The sub-County has two rainfall seasons [21]. The long rains occur between March and May, while the short rains, which are the most significant, start from October to December. The annual rainfall in the area ranges between 100 and 250 mm with an average annual temperature of 29 °C [21]. Livestock production is the main economic activity in the area with nomadic pastoralism being prominent.

Sample size determination and sand fly sampling
Since sand flies for this study were sampled at a single time point in time (cross-sectional sampling), we estimated the sample size, using the prevalence formula, according to Amin et al. [23]. Due to the lack of information on Leishmania infection prevalence in the vectors, we calculated our sample size assuming an expected infection prevalence of 50% to obtain an optimum sample size for the study. Therefore, at 95% confidence level and 5% precision, we estimated that a minimum of 385 sand flies was required to satisfy the study objectives.
Sand flies were sampled from five villages in Merti sub-County (Barsa, Kambi Juu, Korbesa, Malkagala and Matt Arban) using 12 CDC miniature light traps (John W. Hock Co., Gainesville, FL, USA) and castor oil sticky paper traps. The traps were placed in animal sheds and outdoors in areas likely to harbour sand flies such as near the Acacia trees and in chromic vertisols where Ph. orientalis and other sand flies are thought to breed and/ or rest [10]. None of the traps was set indoors. We set the traps at 0.5-1.5 m above the ground in each of the sampling sites from 1800 to 0600 h the following day to constitute one trapping night. This was repeated over a period of 12 days.

Sand fly dissections and morphological species identification
Following each trapping night, sand flies were sorted and washed in 2% detergent to remove the castor oil and setae, followed by antibiotic and antifungal solutions [24]. For all the sand flies, the head and the third last abdominal segment were dissected and cleared in gum chloral hydrate, which also served as the mountant, for morphological species identifications [25]. The abdominal status of the female sand flies was recorded as fed, unfed, or gravid. Furthermore, we examined the midguts of individual female sand flies for the presence The maps were generated using QGIS version 3.0 [22] of live promastigotes. Morphological species identification of the mounted sand fly specimens was based on the external genitalia of males and features of the pharynx, antennae, and spermatheca for females using standard taxonomic keys [26][27][28]. The remaining parts of the dissected female sand flies (i.e. the thorax, wings, legs, and abdomen) were preserved in 70% ethanol and transported under dry ice to the Centre for Biotechnology Research and Development (CBRD), KEMRI, for parasite culture and further morphological identification and the International Centre of Insect Physiology and Ecology (icipe) laboratories for molecular analyses.

Molecular identification of sand fly species
In the laboratory, we homogenised the remaining parts of each sand fly specimen in 180 μl of buffer ATL (QIAGEN, Hannover, Germany) taking care to avoid contamination between the specimens. Genomic DNA was extracted from each homogenate using the DNeasy Blood and Tissue Kit (QIAGEN, Hannover, Germany) according to the manufacturer's recommendations. The extracted DNA was stored at − 20 °C until use.
To validate the morphological species identifications, we amplified the sand fly mitochondrial cytochrome c oxidase subunit I (cox1) gene according to Kumar et al. [29]. The resulting 700-bp cox1 amplicons were purified using the QIAquick PCR purification kit (QIAGEN, CA, USA) according to the manufacturer's recommendations and submitted to the Macrogen (The Netherlands) for sequencing using the forward primer.

Detection of Leishmania and Trypanosoma species in sand flies
We carried out the detections in 57 individual specimens (blood-fed: n = 40; unfed: 17) by PCR amplification of the Leishmania internal transcribed spacer 1 (ITS1) region using L5.8S and LITSR primers [30]. The 20-μl PCR mixtures contained 1× Dream Taq buffer with 2 mM MgCl 2 (Thermo Scientific, USA), 0.25 mM dNTPs mix, 500 nM of each primer, 2 U of Dream Taq DNA polymerase (Thermo Scientific, USA), 5-10 ng of DNA template, and nuclease-free water (Sigma, St. Louis, MO, USA). We included both positive controls [DNA from L. major-Friedlin str. and L. donovani (NLB065) and negative control (nuclease-free water)] in each PCR reaction. We performed all the reactions in a SimpliAmp thermal cycler (Applied Biosystems, Loughborough, UK). The cycling conditions included an initial denaturation for 2 min at 98 °C, followed by 35 cycles of denaturation for 20 s at 95 °C, annealing for 30 s at 53 °C, an extension for 30 s at 72 °C, and a final extension at 72 °C for 5 min. The PCR products were run in 1.5% agarose gel stained with 1× ethidium bromide (Thermo Scientific, USA) and visualised in a GenoPlex gel documentation and analysis system (VWR, USA). The amplicons from positive samples were purified as aforementioned and submitted for sequencing at Macrogen (The Netherlands) using both the forward and reverse primers.

Sand fly, Leishmania, and Trypanosoma sequence and phylogenetic analyses
The chromatograms of all sequences obtained were trimmed and edited using Geneious Prime software (v2020.0) to obtain consensus sequences for each sample. The consensus sequences were aligned using MUS-CLE [31] with homologous sequences identified by sequence similarity searches in GenBank using the Basic Local Alignment Search Tool (BLAST) [32]. We constructed maximum-likelihood phylogenies of sand fly CO1, Leishmania ITS1, and Trypanosoma ITS1 sequence alignments using PhyML version 3.0 [33] employing the general-time-reversible (GTR) sequence evolution model. Tree topologies were estimated over 1000 bootstrap replicates.

Identification of vertebrate hosts represented in sand fly blood meals
We determined the blood-meal sources of engorged sand flies by real-time PCR amplification of the vertebrate mitochondrial cytochrome b (cyt-b) gene followed by high-resolution melting (HRM) analysis as previously described [34]. All reactions were performed in a Rotor-Gene Q real-time PCR thermocycler (QIAGEN, Hannover, Germany) using DNA extracted from the blood of known vertebrate samples as positive controls and nuclease-free water as the negative control. The positive controls included blood from livestock, rodents, and small mammals commonly found around the homestead such as rabbits and rock hyraxes. Livestock included goats, sheep, cow, and camels, while rodents included rats and mice. We also included human DNA extracted from blood obtained from a volunteer in icipe. Swiss mouse, rabbit, and rat blood were sourced from the icipe's Animal Rearing Unit, while blood samples from the other animals were obtained from a slaughterhouse. We identified the vertebrate hosts represented in sand fly blood meals by comparing the melting profiles of the samples to those of the positive controls. Samples with melting profiles that did not match those of the positive controls were purified as described above and submitted for sequencing using the forward primer. The cyt-b chromatograms were edited in Geneious Prime software (v2020.0) and queried against the GenBank database using the NCBI's BLASTn. The selected match was the top hit vertebrate species with the lowest e-value and homology cut-off values of 90-100% as the most likely sand fly hosts.

Statistical analyses
We performed statistical analyses using SPSS (v26) software. Descriptive statistics were used to determine the distribution pattern and frequency of each sand fly species per village. The species abundance was determined as the quantitative counts per village. The Kruskal-Wallis test was used to analyse differences in species distribution between villages.

Molecular identification of sand fly species
We amplified the cox1 region of 57 morphologically identified sand flies consisting of Ph. orientalis, S. clydei, and S. squamipleuris specimens. A BLAST search of the NCBI's database using the cox1 gene sequences from the infected and a few uninfected S. squamipleuris sand flies (GenBank accessions: MT594454-MT594459) showed a high percentage identity (96-99.8%) with those of Sergentomyia species in GenBank. Similarly, the cox1 sequences from Ph. orientalis (GenBank accessions: MT597050-MT597055) indicted a 97-99.7% identity with other Ph. orientalis sequences in GenBank, thus confirming the morphological species identifications. Phylogenetic analysis of the cox1 sequences revealed genus-specific clusters with all the Ph. orientalis and S. squamipleuris sequences separated into different branches (Fig. 2). The S. squamipleuris specimens from this study clustered together with other S. squamipleuris specimens collected from China (GenBank accessions: MF966747; MF966746). However, they segregated into different sub-branches.

Detection of Leishmania and Trypanosoma spp. in sand flies
We did not observe Leishmania promastigotes in the midguts of the dissected female sand flies by microscopy. To improve the sensitivity of Leishmania detection in the samples, we tested a total of 57 sand flies (blood-  specimens. Strikingly, we observed a band of approximately 570 bp in one S. squamipleuris specimen (Additional file 1: Figure S1). We did not detect Leishmania DNA in all Ph. orientalis and S. clydei samples analysed. More so, all the positive samples were collected from Malkagala village. A BLAST search of the NCBI's database using the ITS1 sequence (GenBank accession: MT548852) from one of the positive S. squamipleuris samples revealed a high percentage identity (98.8%) with L. major sequences in GenBank. Similarly, sequences from two of the positive S. squamipleuris samples (GenBank accessions: MT548853-MT548854) showed a 96.9-100% identity with L. donovani sequences in GenBank. In the phylogenetic tree, the L. major and L. donovani ITS1 sequences from this study segregated into the Leishmania major and Leishmania donovani clusters with bootstrap support values of 92% and 99%, respectively (Fig. 3). These clusters contained Leishmania spp. that have been isolated from patients in different regions and whose sequences are available in GenBank.
Unexpectedly, the ITS1 sequence of the 570-bp amplicon (GenBank accession: MT548851) did not match those of Leishmania spp., but rather showed high homology with Trypanosoma spp., suggesting that it belonged to the Trypanosoma genus. A BLAST search analysis of the sequence revealed a 91.6% identity with the ITS1 sequence of Trypanosoma rangeli registered in the GenBank. This sequence was placed into a monophyletic cluster with other trypanosomes isolated from humans and other animals and was unrelated to those isolated from reptiles (e.g. T. grayi). We found the sequence to be closely related to that of T. rangeli isolated from humans than the other Trypanosoma species (Fig. 4), which further suggests the subgenera it belongs to. Although this finding was further corroborated by patristic distance matrix analysis (Additional file 2: Figure S2), the Trypanosoma DNA detected within Sergentomyia squamipleuris segregated into a different monophyletic sub-branch, suggesting that it was novel or genetically uncharacterised.

Sand fly blood-meal source determination
We determined the blood-meal sources of all the engorged sand flies consisting of S. squamipleuris (n = 36) and four Ph. orientalis sand flies. The cytb HRM profiles revealed various vertebrate hosts in sand fly blood meals, including humans (Homo sapiens), rock hyraxes (Procavia capensis), and rodents ( Table 2). Humans were the main blood-meal sources detected exclusively in 66.7% (n = 24) and 50% (n = 2) of the engorged S. squamipleuris and Ph. orientalis, respectively. All the sand flies that were infected with Leishmania major and Leishmania donovani had taken a blood meal exclusively from humans. The presence of mixed feeding was identified based on HRM profiles with multiple peaks compared to the reference controls. We identified mixed feeding in three samples: two Phlebotomus orientalis and one Sergentomyia squamipleuris. One of the Phlebotomus orientalis specimens had taken a blood meal from humans and rock hyraxes while the other had multiple blood meals amongst humans, rock hyraxes, and mice. In contrast, the Sergentomyia squamipleuris specimen had blood meals from humans and mice. We did not characterise blood-meal sources of 11 samples including one sample that we found to be infected with Trypanosoma spp. as the cyt-b amplification failed in these samples. The representative cyt-b sequences from this study have been deposited in the GenBank nucleotide database under the accession numbers: MT568790-MT568795.

Discussion
The main goal of this study was to examine sand fly species with the potential to transmit Leishmania parasites in Merti sub-County and determine their abundance, distribution, and blood-meal sources. We detected L. major, L. donovani, and Trypanosoma spp., for the first time, in Sergentomyia squamipleuris from a VL-endemic area in Kenya. These findings reveal urgent questions to be addressed regarding the dogma that human leishmaniases in the Old World are exclusively transmitted by sand flies of the Phlebotomus genus [1,35]. We identified eight sand fly species from all the study villages. These consisted of one Phlebotomus and seven Sergentomyia species. Phlebotomus orientalis was the only Phlebotomus species sampled in this study and represented one out of the five Phlebotomus subgenera described in Kenya [25]. Phlebotomus orientalis is implicated as the secondary vector of L. donovani in Kenya [10]. However, it was found to occur in relatively low abundance in all the sampling villages. The low numbers of Ph. orientalis in this study corroborated previous reports in Kenya that suggest that the species do not occur in large numbers in VL endemic areas [10]. The low abundance of Ph. orientalis could be attributed to very few cracked vertisols in the area during the sampling period. Since we conducted the sampling after the long rainy season, the soil had not dried enough to form deep cracks which act as breeding sites for the sand fly species [28]. Sand flies of the Sergentomyia subgenus were the predominant species, with S. squamipleuris being the most abundant, accounting for 78.71% and present in all the study villages, followed by S. clydei (10.46%). We found the distribution of sand fly species across the sampling villages to be significantly different with the highest sand fly density being recorded in Kambi Juu, while Korbesa had the lowest sand fly density.
Phylogenetic analysis of the sand fly cox1 sequences separated Ph. orientalis and S. squamipleuris into genusspecific clusters, confirming the morphological species identifications. The S. squamipleuris specimens from this study and that collected from China segregated into different sub-branches, which reflects the genetic isolation due to geography. The high prevalence of Leishmania spp. and the detection of Trypanosoma spp. in S. squamipleuris sand flies suggest the possible involvement of the sand fly in the transmission of Leishmania spp. and Trypanosoma spp. in Merti.
Demonstrating Leishmania infections in sand flies is a prerequisite for vector incrimination. The vectorial role of a sand fly species is epidemiologically suspected if the species is predominant in a leishmaniasis focus and exhibits an anthropophilic and anthropophagic behaviour [15]. This hypothesis is supported if the same species is found infected with live transmissible promastigotes like those isolated from humans and other vertebrate reservoirs. In this study, we did not observe Leishmania promastigotes in the midguts of all the dissected female sand flies by microscopy. Although this method is used as the gold standard for demonstrating Leishmania infections in sand flies [36], it has low sensitivity, which reduces with a decrease in parasite burden in the flies. The low sensitivity could explain why we did not observe live infections in the infected flies. Using the ITS1-PCR followed by sequencing, we detected, for the first time in Kenya, Leishmania spp. and Trypanosoma spp. DNA in four samples of S. squamipleuris. Leishmania major and Trypanosoma species were detected in one specimen each, while two of the samples were found to harbour L. donovani parasites.
Comparison of Leishmania spp. identified in sand flies with those isolated from humans is crucial in predicting the risks of disease transmissions in endemic areas. Phylogenetic analysis of the ITS1 sequences of the Leishmania spp. identified in this study revealed that the sequences were closely related to other Leishmania species isolated from humans. The L. major sequence segregated into the L. major cluster with bootstrap support values of 100% and was closely related to L. major sequence isolated from a CL patient in Ghana [37]. Similarly, we found the L. donovani sequence to be closely related to other L. donovani sequences isolated from humans. However, the two sequences were separated into monophyletic clusters, which may suggest an incomplete lineage sorting. The detection of L. major and L. donovani, similar to those infecting humans, in S. squamipleuris sand flies from Merti focus is a novel finding and corroborates other studies in Kenya [19] and elsewhere [13,15,16,18], in which sand flies of this genus were found to be naturally infected with Leishmania spp. pathogenic to humans.
To date, to our knowledge, there is no published report on the role of S. squamipleuris as a vector of Leishmania or Trypanosoma spp. in Kenya. Our results, in addition to the previous reports, further suggest that S. squamipleuris could be involved in the transmission cycle of Leishmania parasites in Merti, where there were low numbers of Phlebotomus species and none of these was found to be infected with Leishmania parasites. Although Sadlova Table 2 Sand fly blood meal sources in Merti sub-County determined by real-time PCR of the vertebrate cyt-b gene followed by HRM analysis ND: blood-meal sources were not determined; the number in brackets indicates the number of blood-fed sand flies that were positive for Leishmania spp. and Trypanosoma spp.

Species
Humans  [18]. Further investigations are needed to determine whether Sergentomyia sand flies can support the cyclic development of Trypanosoma spp. that are pathogenic to humans and other animals. Analysis of the sand fly blood-meal sources revealed humans as the main sand fly blood-meal hosts in Merti sub-County. The significant preference for humans (65%) further suggests a high risk of disease transmissions in the study site and could explain the recurrent outbreaks and the rising number of VL cases in the area. Most of the blood-fed sand flies were S. squamipleuris (90%), the majority of which had taken blood meals exclusively from humans. Only two of the S. squamipleuris specimens had mixed blood meals in their gut. These results aligned well with other studies in Sudan where sand flies of the Sergentomyia genus were reported as a common human-biting species [13]. Of the 24 S. squamipleuris sand flies that fed on humans, two were infected with L. donovani, one with L. major and one with Trypanosoma spp. These findings challenge the dogma that human leishmaniases in the Old World are exclusively transmitted by sand flies of the Phlebotomus genus [1,35]. Although we identified human blood meals in two Ph. orientalis samples and mixed feeding in two additional samples of the same species, none of these samples was found to be infected with Leishmania parasites. Our results illustrate the potential role of S. squamipleuris in the transmission of Leishmania spp. in Kenya, providing useful information to guide in addressing the epidemiology of the disease including control.
Other vertebrate hosts represented in sand fly blood meals included rock hyrax (Procavia capensis) and mouse (Mus musculus). Since both Ph. orientalis and S. squamipleuris do not feed exclusively on humans, there is a possibility of zoonotic transmission of leishmaniasis and other pathogens in this VL focus. Characterisation of blood-meal sources failed in 11 samples. This could be due to the degradation of the cyt-b target, highlighting the importance of using more than one genetic marker for the analyses of host preferences.
Detection of both L. donovani and L. major in S. squamipleuris that mainly fed on humans further suggests that this species may be a potential permissive vector of the Leishmania species. In most permissive vectors of Leishmania spp. such as Lutzomyia longipalpis and Ph. arabicus, the attachment of Leishmania parasites to the midgut is independent of the midgut lipophosphoglycan (LPG). Whether such mechanisms also apply to the sand flies of the Sergentomyia species in which more than one Leishmania spp. pathogenic to humans have been identified remains to be determined. Moreover, further studies are needed to determine the role of S. squamipleuris in the transmission cycle of L. donovani and L. major parasites by determining the developmental stages of the parasites in individual field-collected sand flies and transmission experiments or through evaluating Leishmania developmental stage-specific gene expression.

Conclusions
We demonstrate, for the first time, the detection of L. major, L. donovani, and Trypanosoma spp. in S. squamipleuris sand flies from a VL focus in Merti sub-County, eastern Kenya. This sand fly species was the most abundant in the study area and exhibited anthropophilic and anthropophagic behaviour, which suggests its potential involvement in Leishmania transmission in the sub-County. Identification of both L. major and L. donovani in S. squamipleuris further suggests that it may be a potential permissive vector of both parasites, a finding that needs further investigations. Identification of Trypanosoma spp. in S. squamipleuris may indicate mechanical transmission, whose efficiency should be investigated. Analysis of sand fly blood-meal sources revealed the possibility of zoonotic transmission of leishmaniasis and possibly other pathogens in Merti sub-County. Further studies are needed to incriminate the vector and establish vector competence and reservoir hosts of Leishmania parasites in this area.