Insecticide resistance to insect growth regulators, avermectins, spinosyns and diamides in Culex quinquefasciatus in Saudi Arabia

Background Culex quinquefasciatus is not only a biting nuisance but also an important vector of fatal diseases. In Saudi Arabia, management measures to control this mosquito and thereby prevent associated disease transmission have focused on insecticides. Nevertheless, information on the resistance status of C. quinquefasciatus is insufficient, especially concerning insecticides containing novel classes of insecticides. Methods We evaluated six insecticides belonging to four classes of insecticides (insect growth regulators [3], avermectins [1], diamides [1] and spinosyns [1]) for toxicity and resistance in eight C. quinquefasciatus populations (from Ishbiliya, Al-Masfa, Al-Masanie, Al-Washlah, Al-Nakhil, Irqah, Al-Suwaidi and Al-Ghanemiya) following World Health Organisation protocols. Results Resistance status ranging from susceptibility/low resistance to high resistance, in comparison with the susceptible strain, was detected for cyromazine in the eight C. quinquefasciatus populations: Ishbiliya (resistance ratio [RR] = 3.33), Al-Masfa (RR = 4.33), Al-Masanie (RR = 3.67), Al-Washlah (RR = 2.33), Al-Nakhil (RR = 5.33), Irqah (RR = 7.00), Al-Suwaidi (RR = 21.33) and Al-Ghanemiya (RR = 16.00). All C. quinquefasciatus populations exhibited a high level of resistance to diflubenzuron (RR = 13.33–43.33), with the exception of Al-Nakhil which exhibited moderate resistance (RR = 10.00). Susceptibility/low resistance to high resistance was observed for triflumuron in the eight C. quinquefasciatus populations: Ishbiliya (RR = 0.50), Al-Ghanemiya (RR =  3.00), Al-Suwaidi (RR =  10.00), Al-Masfa (RR =  5.00), Al-Masanie (RR =  10.00), Al-Nakhil (RR =  5.00), Irqah (RR =  5.00) and Al-Washlah (RR =  15.00). Susceptibility/low resistance was assessed for abamectin, chlorantraniliprole and spinosad in all C. quinquefasciatus populations, with RR ranges of 0.25–3.50, 0.17–2.19, and 0.02–0.50, respectively. However, the population collected from Irqah showed high resistance to chlorantraniliprole (RR = 11.93). Conclusions The detection of widespread resistance to insect growth regulators in C. quinquefasciatus highlights an urgent need to establish integrated vector management strategies. Our results may facilitate the selection of potent insecticides for integrated vector management programmes for C. quinquefasciatus. Graphical Abstract


Background
Culex quinquefasciatus is not only a biting nuisance to its hosts [1,2] but also a significant insect vector of various parasites, including West Nile fever, lymphatic filariasis and Japanese encephalitis [3][4][5]. Culex quinquefasciatus can breed in various aquatic habitats, including mangrove swamps, fresh or salt water marshes, stream and river edges and temporary standing rain water [6][7][8], and human settlements, agricultural lands and livestock farms with inadequate hygienic practices/facilities are also ideal breeding habitats.
Management measures, such as the use of genetic manipulation, sterile insect techniques, eradication of breeding places, chemical insecticides and natural biological agents, have been used to control insect vectors, including C. quinquefasciatus, worldwide [1,9,10]. Of these, the management of C. quinquefasciatus to prevent associated parasite transmission has focused on insecticides as a rapid control measure [11,12]. At the present time, avermectins, diamides and spinosyns, are being used widely to control the larvae of C. quinquefasciatus and other insect vectors worldwide [1,13,14]. However, the extensive and widespread use of these insecticides has led to resistance in C. quinquefasciatus in different parts of the world [1,[15][16][17][18][19] and, in adddition, has caused environmental pollution, increases in the preventive costs of chemical control and destroyed nontarget organisms [20][21][22]. Taken together, these factors necessitate the use of integrated vector management programmes against C. quinquefasciatus.
The IGRs cyromazine, diflubenzuron and triflumuron are currently the most effective larvicides for controlling mainly dipteran pests, including mosquitoes [13,[23][24][25]. Cyromazine is a molting disruptor, whereas diflubenzuron and triflumuron are chitin synthesis inhibitors. Abamectin (an avermectin) and spinosad (a spinosyn) are biorational insecticides, with the former being a glutamate-gated chloride channel allosteric modulator and the latter a nicotinic acetylcholine receptor allosteric modulator [26]. Chlorantraniliprole is an anthranilic diamide insecticide; it acts as a ryanodine receptor modulator in insect muscles [26], causing the uncontrolled release of calcium ions that leads to feeding cessation, lethargy, muscle paralysis and ultimately death [27,28]. Because of their low mammalian toxicity and low hazard threat to the target's natural enemies, these insecticides are good candidates for the management of various insect pests, including mosquitoes [29][30][31].
Mosquito control programmes in Riyadh, Saudi Arabia rely mainly on chemical control. Consequently, there is always the possibility of the development of insecticide resistance, which would, for example, reduce the effectiveness of chemical control against C. quinquefasciatus. It is crucial to know the resistance status of C. quinquefasciatus to newly developed insecticides before their widespread use in Riyadh (Fig. 1). Hence, we evaluated the toxicity and resistance levels of six novel insecticides in the IGR, avermectin, diamide, and spinosyn classes against eight C. quinquefasciatus larval populations from different areas in Riyadh, Saudi Arabia. Baseline susceptibility data from this study will help in the design of appropriate and effective strategies for controlling C. quinquefasciatus.

Culex quinquefasciatus populations
Approximately 200 C. quinquefasciatus larvae at mixed developmental stages were collected from standing water or from containers at each of the eight different study locations in the environs of Riyadh, Saudi Arabia; these sites were designated ISH, SUW, GHN, MSF, MSN, NKL, WSH and IRQ ( Fig. 1; Table 1). The locations were selected on the basis that there was a high chance they had been exposed to pesticides. Each population was collected and maintained separately. After collection, larvae of each population were transferred to alternative plastic containers (4 l) in the laboratory and provided cattle food ad libitum for feeding until pupation. The emerged adults were transferred into cages (30 × 30 cm), and cotton wicks soaked in 10% sugar solution were provided as food. The cotton wicks were moistened every 2 days and replaced when they became dirty. A restrained pigeon  Table 1 for abbreviations of study/ population collection sites with keel feathers removed was provided for 12 h overnight for blood-feeding during each oviposition period. Plastic cups (500 ml) containing tap water were placed in the adult cages to receive egg masses and to obtain uniform F 1 populations. The plastic cups were then removed and the eggs transferred into plastic containers. Hatched larvae were provided with cattle food ad libitum and third-instar larvae were used for the bioassay. All populations were maintained separately in the laboratory at 27℃ ± 2 °C, 65% ± 5% humidity and under a 12:12 h (light: dark) photoperiod.
The susceptible reference strain (designated as SUS) was obtained from the High Institute of Public Health, Alexandria University, Egypt, in 1990 and has been maintained since then under the above-mentioned protocol with no exposure to any kind of chemicals.

Larva bioassays
The bioassays of the insecticides against C. quinquefasciatus larvae were performed following the protocol proposed by the World Health Organisation [32]. For each insecticide, five concentrations that caused mortality ranging from > 0% to < 100% were prepared in tap water by serial dilution from a stock solution (1000 ml). A fresh stock solution was prepared for each replication, and assays were performed at different times to ensure true replication [33]. Third-instar larvae from each pooled population were kept in plastic cups containing 400 ml of the test solution. Insecticide dilution bioassays were performed four times, with 10 larvae per replicate and a total of 240 larvae in each bioassay. A total of 40 larvae (four replicates/9 larvae in each) were used in the control treatment. All bioassays were conducted and maintained under the above-mentioned laboratory conditions. Mortality was recorded after 48 h for the abamectin, chlorantraniliprole and spinosad treatment groups. For the three IGR groups, mortality data were recorded after adult emergence, with pupae failing to emerge as adults considered to be dead.

Data analyses
The bioassay data were analysed using POLO Plus software [34] to determine the median lethal concentration (LC 50 ), 95% fiducial limits (FLs), standard error, and chi-squared (χ 2 ) test. Using the Abbott [35] formula, mortalities were corrected when needed by reference to mortality in the control treatment. LC 50 values were considered to be significantly different if their 95% FLs did not overlap [36]. Resistance ratios (RRs) were calculated as: LC 50 for the field population/LC 50 for the susceptible strain. The RRs were classified as follows: RR < 5 indicated susceptibility/low resistance; RR = 5-10 indicated moderate resistance and RR > 10 indicated high resistance [32,37].

Resistance to IGRs
The LC 50 and RR values for the IGRs cyromazine, diflubenzuron and triflumuron for the eight C. quinquefasciatus field larval populations are reported in Table 2.

Resistance to abamectin, chlorantraniliprole and spinosad
The LC 50 and RR values for abamectin, chlorantraniliprole and spinosad for the eight C. quinquefasciatus field larval populations are reported in Table 3.
The LC 50 for abamectin ranged from 0.002 to 0.028 µg/ ml, and the RRs ranged from 0.25 to 3.50 for all eight field populations, indicating that all populations were susceptible/showed low resistance to abamectin.
The LC 50 for chlorantraniliprole ranged from 0.005 to 0.346 µg/ml and the RRs ranged from 0.17 to 11.93. Susceptibility/low resistance was observed in all field populations with the exception of the IRQ population, which showed high resistance to chlorantraniliprole.
The LC 50 for spinosad ranged from 0.0002 to 0.006 µg/ ml, and the RRs ranged from 0.02 to 0.50, indicating that all field populations exhibited susceptibility/low resistance to spinosad.

Discussion
Information on the susceptibility status of insect vectors to commonly used insecticides is a key aspect in the selection of the most appropriate and effective insecticide [13,38,39]. In this study, the eight C. quinquefasciatus populations showed varying levels of resistance, ranging from low to high, against cyromazine. Cases of resistance to cyromazine have been documented in various medical and veterinary pests around the world. For example, different levels of cyromazine resistance have been found in the house fly Musca domestica (RR = 4.8 [40], RR = 62.5 [41], RR = 5.6 [42], RR = 18.0 [43,44] and RR = 2.9 [13]) and in the Australian sheep blowfly Lucilia cuprina (RR = 12.8 [45]).
In the present study, all test populations of C. quinquefasciatus exhibited a high level of resistance to the IGR diflubenzuron; in contrast, for the IGR triflumuron, one population showed high resistance, five showed moderate resistance and two showed susceptibility/low resistance. Diflubenzuron has been found to be an effective IGR against the stable fly Stomoxys calcitrans and the mosquitoes Aedes aegypti and Aedes albopictus [23][24][25]. However, a high level of diflubenzuron resistance was found in M. domestica (RR = 120) [40], in the sheep body louse Bovicola ovis (RR = 90) [46] and in the mosquito Culex pipiens (RR = 128) [47]. Similarly, triflumuron resistance has been well documented in B. ovis (RR = 93.8) [46]. Triflumuron has been found found to be an effective IGR against M. domestica [13,44] and against C. quinquefasciatus [48].
Susceptibility/low resistance to the avermectin abamectin was detected in the C. quinquefasciatus populations in the present study. Previously, resistance to abamectin has been documented in M. domestica (RR = 5.9-94.4) [22,49].
We also detected susceptibility/low resistance to diamide chlorantraniliprole in all C. quinquefasciatus populations from the tested regions, with the exception of the IRQ population which showed high resistance (RR = 11.93) when compared with the susceptible strain. Previously, field-evolved resistance to chlorantraniliprole has been reported in M. domestica (RR = 36) [28]. In this study, susceptibility/low resistance to the spinosyn spinosad was detected in the C. quinquefasciatus populations from the tested regions. Field-evolved resistance to spinosad has been reported in M. domestica (RR = 7.2) [22].
The evolution of insecticide resistance arises when a population has been intensively exposed to a pesticide, resulting in the elimination of susceptible individuals and the survival of resistant ones [21]. In this study, susceptibility/low resistance to abamectin, chlorantraniliprole and spinosad is an interesting finding and could could reflect the low or absence of usage of these insecticides against mosquitoes in Saudi Arabia, although insecticides with such novel formulations are commonly used against agricultural insect pests in Saudi Arabia.
This study highlights high levels of resistance in C. quinquefasciatus to diflubenzuron and triflumuron, possibly due to: (i) cross-resistance arising from concurrent usage of conventional insecticides like organophosphates and pyrethroids [13]; (ii) target-site mutations or involvement of metabolic enzymes [47,[50][51][52]; (iii) extensive usage of these insecticides in mosquito control programmes [53]; (iv) detoxification by enzymes [54,55]; and/or (v) lack of effective and successful resistance management strategies [21]. Resistance to cyromazine is emerging in some C. quinquefasciatus populations, so this insecticide should be used rotationally in those locations where resistance has developed to delay further development of resistance. Nevertheless, further studies on cross-resistance patterns, metabolic mechanisms and target-site resistance mutations will confirm the exact phenomena. Laboratory selection of C. quinquefasciatus under the influence of diflubenzuron is currently under evaluation to explore cross-resistance and the mechanisms of resistance.

Conclusions
In conclusion, the evidence from this study of resistance to the IGRs cyromazine, diflubenzuron and triflumuron in C. quinquefasciatus indicates the potential lack of systematic management practices in Saudi Arabia. We believe that it is necessary to urgently establish effective resistance management strategies to delay the further development of resistance to these IGRs. Resistance management strategies, including the rotational use of potent insecticides, the integration of cultural practices, such as removal of breeding places, and the use of biological control agents [9], should be applied for the management of C. quinquefasciatus to minimise over-reliance on insecticides. The susceptibility/low resistance of C. quinquefasciatus larvae to abamectin, chlorantraniliprole, and spinosad suggests that these insecticides retain good potency and hence should be used rotationally with IGRs to sustain their efficacy. Periodic monitoring of resistance to these insecticides should be continued to detect any further increases in resistance. The findings of this study can serve as a reference in future monitoring efforts of C. quinquefasciatus insecticide susceptibility.