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Table 2 PCR primers and conditions

From: Isolation and preservation of schistosome eggs and larvae in RNAlater® facilitates genetic profiling of individuals

 

Primers

DNA region

Forward

Reverse

COX1

Schisto5'

Schisto3'

 

TCTTTRGATCATAAGCG

TAATGCATMGGAAAAAAACA

ITS

ITSF

TAACAAGGTTTCCGTAGGTGAA

ITSR

TGCTTAAGTTCAGCGGGT

  1. 25 μl PCR reactions were carried out using Ready-to-go PCR Beads (Amersham) and 10 pmol of each primer for the DNA region to be amplified as above. Thermal cycling conditions were: 2 min denaturing at 95°C: 40 cycles of 30 s at 95°C, 30 s at 40°C, 2 min at 72°C; followed by final extension period of 7 min at 72°C. Positive amplification was checked by 0.8% Gel Red agarose gel electrophoresis of 3 μl of each amplicon, which were visualised using a UVP gel doc system (Fig 1).
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Parasites & Vectors

ISSN: 1756-3305

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