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Figure 2 | Parasites & Vectors

Figure 2

From: A luciferase based viability assay for ATP detection in 384-well format for high throughput whole cell screening of Trypanosoma brucei brucei bloodstream form strain 427

Figure 2

Combination of cell concentrations of T.b.brucei and reagent volumes for signal detection when a 384-well plate inoculated directly from flask culture dilutions and detected by the luciferase ATP detection assay. Fifty-five μL of T.b.brucei cell densities ranging from 1-3 × 106 cells/mL were transferred from a flask culture in to a 384 well plate and detected with CellTiter-Glo. Twenty-five, 20, 15 and 10 μL volumes of CellTiter-Glo were compared for signal detection and reproducibility.

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Parasites & Vectors

ISSN: 1756-3305

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