Table 2 Gene identification, primers, gene region amplified, amplicon size and PCR annealing temperature.
Gene identification (purpose) | TC number* or GenBank identification | Forward primer (5'-3') | Reverse primer (5'-3') | Size (bp) | Annealing temperature (°C) |
|---|---|---|---|---|---|
Imnp (full-length) | TC12157 | gcagaattaactatcgcaaa | tttgtgtggtggaaatgaa | 928 | 58 |
Imnp (dsRNA synthesis) | TC12157 | agacttcacgaatcacaa | taagaagaaaggaaaagttg | 380 | 58 |
Imnp (real-time PCR) | TC12157 | ggttacttgtgtggttgt | gtgtggtggaaatgaat | 64 | 60 |
Spi (full-length) | TC9311 | caattcatcctgtgtattctcg | aacccattcgcctgttacg | 1652 | 58 |
Spi (dsRNA synthesis) | TC9311 | caattcatcctgtgtattctcg | caacaacttcgtgactct | 396 | 58 |
Spi (real-time PCR) | TC9311 | taatagcgagggaagtag | caccaaggagaccatttc | 135 | 60 |
Lpc (full-length) | TC8123 | accccgaatccctgtgtt | cagtgccgaccaaagtaagg | 731 | 58 |
Lpc (dsRNA synthesis) | TC8123 | gagaagataggaaatgtgta | tctctgttgtagacgaagt | 379 | 58 |
Lpc (real-time PCR) | TC8123 | aaggagggaagaatacac | ctcaagtttcaaggacag | 134 | 60 |
Actin (real-time PCR) | AY255624 | tcctctccttccttctac | aaagttctgttcgtcgt | 103 | 60 |
tubulin (real-time PCR) | AA257910 | tgtgccccgtgccgtattt | ggcaccagagcgaacct | 64 | 60 |
G6PDH (real-time PCR) | EU595881 | ttctctctctcctcagtg | gaagatgtgttgttgtcc | 88 | 60 |
PHGPx (real-time PCR) | DQ180067 | accaacaagaactacacg | cttctttatgtctgcctca | 131 | 60 |