Figure 1

CTL-PDDVs induced the cytotoxicity effect and produced antibody. (A) Effect of C-PDDV vaccination on cytotoxicity. Seven days after the last C-PDDV, 18K-PDDV, or PBS immunization epitope-specific cytotoxic activity was measured by incubating murine spleen effector cells or p815 target cells with either C1-, C2-, or C3-18K fusion peptides, 18K control peptide, or medium only, then mixed cells at E:T rations ranging from 1:10 to 1:100. The CTL activity of the cells was tested using Na₂[⁵¹Cr]O₄ assay. Data are expressed as the mean ± SD (n = 6 per group) of 18 mice from three independent experiments performed in triplicate wells. (B) Serum antibody subtype profile following C-PDDV vaccination. Whole IgG, IgG1, and IgG2a responses to SWA (0.1 mg/ml) following vaccination with C-PDDV formulations, or controls were measured by ELISA. Data are expressed as the mean ± SD (n = 6 per group) of 18 mice from three independent experiments performed in triplicate wells. * P < 0.05 and ** P < 0.01, compared to the 18K-PDDV and PBS groups. (C-D) Cytokine profile analysis following C-PDDV vaccination. IFN-γ (C) and IL-4 (D) production of splenocytes harvested from every vaccination group were determined by culturing in triplicate. In 96-well plates, 10⁶cells/well were cultured for 48 h in 200 μl of media in the presence of C1-, C2-, C3-18K fusion peptide (10 μg/ml), 18K (10 μg/ml), or media alone. Supernatants were collected after 48 h of culture for cytokine detection. Bars show the mean ± SD (n = 6 per group) of 18 mice from three independent experiments performed in triplicate wells.