Bartonella vinsonii subsp. berkhoffii and Bartonella henselae bacteremia in a father and daughter with neurological disease

Table 3 Serological, culture and molecular test results for the household pets

	*Bartonella* IFA Reciprocal Titers			PCR/DNA Sequencing Results
Pet Designation	*B. henselae*	*Bvb* Genotype II	*Bvb* Genotype III	Direct Extraction	BAPGM Enrichment Culture	Subculture Isolate
JE Cat	512	128	64	Bh H1	Bh H1	Bh H1
CO Cat	<16	128	<16	Bh H1	Bh H1	Bh H1
SN Cat	256	128	64	Neg	Neg	NIO
JA Cat	2048	2048	2048	Bh H1	Bh H1	Bh H1
TO Dog	<16	<16	<16	Neg	Neg	NIO
EM Dog	<16	<16	<16	Neg	Neg	NIO

Neg = DNA was not amplified using Bartonella 16S-23S intergenic spacer (ITS) primers.
NIO = No isolate obtained by subculture following BAPGM (Bartonella alpha Proteobacteria growth medium) pre-enrichment culture.
Bh H1 = Bartonella henselae ITS Houston I like strain by DNA sequencing

ISSN: 1756-3305