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Figure 1 | Parasites & Vectors

Figure 1

From: Characterization of proteinases from the midgut of Rhipicephalus (Boophilus) microplus involved in the generation of antimicrobial peptides

Figure 1

Purification of aspartic proteinases from tick midgut homogenates. Purification was accomplished in three chromatographic steps: anion exchange chromatography (A), hydrophobic interaction chromatography (B) and pepstatin affinity chromatography (C) in FPLC system. Enzyme activity was assayed with SF 29-35 (-o-). Absorbance was monitored at 280 nm (- -). NaCl gradients (---) were developed as described in Methods. (D) SDS-PAGE of active fraction eluted from the pepstatin affinity chromatography (see also Additional File 1).

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Parasites & Vectors

ISSN: 1756-3305

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