Table 1 Primers used in identification of Theileria orientalis types

SSU rRNA T. orientalis Type specific Forward Primer

Ts-Ikeda

5'-AAGGATCCGTCTCTGCTACCGCCGC-3'

826bp (Kubota et al., 1996)

Ts-Chitose

5'-GCGGATCCTCATCGTCTCTGCAACT-3'

831bp "

Ts-Buffeli

5'GCGGATCCGCTCTGCAACCGCAGAG-3'

825bp "

Type specific Universal Reverse Primer

Ts-R

5'-TGTGAGACTCAATGCGCCTA-3'

MPSP ( Targeting entire Theileria orientalis group)

MPSP Forward Primer

5'-CTTTGCCTAGGATACTTCCT-3'

776bp ( Ota et al., 2009)

MPSP Reverse Primer

5'-ACGGCAAGTGGTGAGAACT-3'

SSU (Theileria orientalis)

SSU rRNA Forward Primer

5'-ACCTGGTTGATCCTGCCAGT-3'

1800bp (Chae et al., 1998)

SSU rRNA Reverse Primer

5'-TAGGAAGACGTCCAAGTGGAATG-3'

SSU rRNA Internal Primers (F&R)

F-5' ATTGGAGGGCAAGTCTGGTG-3'

700bp (This study)

R-5'- CTCTCGGCCAAGGATAAACTCG-3'