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Table 1 Estimated numbers of bacterial cells in whole animals and dissected midguts

From: Use of the checkerboard DNA-DNA hybridization technique for bacteria detection in Aedes aegypti (Diptera:Culicidae) (L.)

Insect samples

Asaia sp.

Chryseobacterium sp.

Klebsiella sp.

Serratia sp.

A. aegypti L1 (w)

5.7 × 105

<105

3.2 × 105

1.8 × 105

A. aegypti L2 (w)

<105

<105

<105

1.1 × 105

A. aegypti P1 (w)

1.6 × 105

<105

1.4 × 105

1.5 × 105

A. aegypti P2 (w)

<105

<105

<105

1.5 × 105

A. aegypti A1 (w)

2.5 × 105

<105

1.2 × 105

1.8 × 105

A. aegypti A2 (w)

3.4 × 105

<105

1.4 × 105

3.1 × 105

A. aegypti L1 (mg)

3.0 × 105

<105

2.8 × 105

2.0 × 105

A. aegypti L2 (mg)

5.1 × 105

<105

4.4 × 105

2.9 × 105

A. aegypti A1 (mg)

< 105

<105

<105

N.D.

A. aegypti A2 (mg)

N.D.

N.D.

<105

<105

A. aegypti A3 (mg)

N.D.

N.D.

<105

N.D.

L. longipalpis (mg)

N.D.

N.D.

<105

<105

D. melanogaster (mg)

<105

N.D.

2.1 × 105

<105

B. hygida (mg)

1.4 × 106

N.D.

8.1 × 105

7.2 × 105

A. mellifera (mg)

3.1 × 105

N.D.

1.6 × 105

<105

  1. The images shown in Figure 2 were digitized and the numbers of bacterial cells in individual whole A. aegypti and dissected insect midguts of A. aegypti, Lutzomyia longipalpis, Drosophila melanogaster, Bradysia hygida and Apis mellifera were estimated using the Image-Quant TL software (GE Healthcare UK), as described in Additional file 1.
  2. w, whole individual; mg, midgut; ND, not detected.
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Parasites & Vectors

ISSN: 1756-3305

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