Figure 5

HLGP is a carbohydrate-binding protein - HA and SPR. A) Hemagglutination activity of purified HLGP. C - negative control (no hemagglutination activity), H - serial two-fold dilution of electroeluted HLGP. Hemagglutination activity is visible in dilutions 2× to 16×. B) Carbohydrate-binding specificity of purified HLGP assessed by SPR on a chip with four channels arranged sequentially as galactose-fucose/mannose-mannose-fucose. Red line corresponds to signal from the channel with immobilized galactose, green to channel with immobilized fucose and mannose, blue to channel with immobilized mannose and purple to channel with immobilized fucose. The fucose/mannose channel contained half amount of the corresponding carbohydrates and serves as control. Lines correspond to SPR response in real-time (not normalized for the sequential flow through channels). Galactose showed the strongest binding by HLGP, some binding was observed also for mannose. Fucose was not recognized by the protein.